Overview

  • Product nameAnti-IDH2 antibody
    See all IDH2 primary antibodies
  • Description
    Mouse monoclonal to IDH2
  • Tested applicationsSuitable for: IHC-P, Flow Cyt, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human, Recombinant Fragment
  • Immunogen

    Recombinant fragment: HYREHQKGRP TSTNPIASIF AWTRGLEHRG KLDGNQDLIR FAQMLEKVCV ETVESGAMTK DLAGCIHGLS NVKLNEHFLN TTDFLDTIKS NLDRALGR, corresponding to amino acids 354-452 of Human IDH2

Properties

Applications

Our Abpromise guarantee covers the use of ab55271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

Target

  • FunctionPlays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex.
  • Involvement in diseaseD-2-hydroxyglutaric aciduria 2
    Glioma
    enetic variations are associated with cartilaginous tumors such as enchondroma or chondrosarcoma.
  • Sequence similaritiesBelongs to the isocitrate and isopropylmalate dehydrogenases family.
  • Post-translational
    modifications
    Acetylation at Lys-413 dramatically reduces catalytic activity. Deacetylated by SIRT3.
  • Cellular localizationMitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • D2HGA2 antibody
    • ICD-M antibody
    • IDH antibody
    • IDH2 antibody
    • IDHM antibody
    • IDHP_HUMAN antibody
    • IDP antibody
    • IDPM antibody
    • Isocitrate dehydrogenase [NADP], mitochondrial antibody
    • Isocitrate dehydrogenase 2 (NADP+), mitochondrial antibody
    • mNADP-IDH antibody
    • NADP(+)-specific ICDH antibody
    • Oxalosuccinate decarboxylase antibody
    see all

Anti-IDH2 antibody images

  • IDH2 antibody (ab55271) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human colon.
  • Western blot against tagged recombinant protein immunogen using ab55271 IDH2 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa
  • ICC/IF image of ab55271 stained Mcf7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55271, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-IDH2 antibody (ab55271)

    Lane 1 : IDH2 in transfected 293T cell line
    Lane 2 : Non-transfected lysate
  • Overlay histogram showing MCF7 cells stained with ab55271 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55271, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-IDH2 antibody (ab55271)

This product has been referenced in:
  • Terunuma A  et al. MYC-driven accumulation of 2-hydroxyglutarate is associated with breast cancer prognosis. J Clin Invest 124:398-412 (2014). WB . Read more (PubMed: 24316975) »
  • Akbay EA  et al. D-2-hydroxyglutarate produced by mutant IDH2 causes cardiomyopathy and neurodegeneration in mice. Genes Dev 28:479-90 (2014). WB . Read more (PubMed: 24589777) »

See all 11 Publications for this product

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear the customer has had difficulty obtaining satisfactory results from this IDH2 antibody.

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Merci de nous avoir contactés. L'anticorps anti-IDH2 ab55271 a récemment été testé sur un lysat de cellules tranfectées. Une bande de 51 kDa, correspondant à la IDH2, a été observée. L'agent bloquant utilisé est le lait à 5%. La fiche techniqu...

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