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Overview

  • Product nameAnti-IDH2 antibody
    See all IDH2 primary antibodies
  • Description
    Rabbit polyclonal to IDH2
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow, Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human IDH2.

    Read Abcam's proprietary immunogen policy

  • Positive control
    • This antibody gave a positive signal in Human Heart, Skeletal Muscle, Liver and Brain Tissue Lysates, and in HepG2, DU 145 and SHSY5Y whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab94359 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 51 kDa).
ICC/IF Use a concentration of 1 µg/ml.

Target

  • FunctionPlays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex.
  • Involvement in diseaseD-2-hydroxyglutaric aciduria 2
    Glioma
    enetic variations are associated with cartilaginous tumors such as enchondroma or chondrosarcoma.
  • Sequence similaritiesBelongs to the isocitrate and isopropylmalate dehydrogenases family.
  • Post-translational
    modifications    Acetylation at Lys-413 dramatically reduces catalytic activity. Deacetylated by SIRT3.
  • Cellular localizationMitochondrion.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • D2HGA2 antibody
    • ICD-M antibody
    • IDH antibody
    • IDH2 antibody
    • IDHM antibody
    • IDHP_HUMAN antibody
    • IDP antibody
    • IDPM antibody
    • Isocitrate dehydrogenase [NADP], mitochondrial antibody
    • Isocitrate dehydrogenase 2 (NADP+), mitochondrial antibody
    • mNADP-IDH antibody
    • NADP(+)-specific ICDH antibody
    • Oxalosuccinate decarboxylase antibody
    see all

Anti-IDH2 antibody images

  • Western blot - Anti-IDH2 antibody (ab94359)
    Western blot - Anti-IDH2 antibody (ab94359)


    Predicted band size : 51 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: IDH2 knockout HAP1 cell lysate (20 µg)
    Lane 3: K562 cell lysate (20 µg)
    Lane 4: HepG2 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab94359 observed at 47 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab94359 was shown to recognize IDH2 when IDH2 knockout samples were used, along with additional cross-reactive bands. Wild-type and IDH2 knockout samples were subjected to SDS-PAGE. ab94359 at a concentration of 1ug/mL and ab8245 (loading control to GAPDH) diluted to 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Western blot - IDH2 antibody (ab94359)
    Western blot - IDH2 antibody (ab94359)
    All lanes : Anti-IDH2 antibody (ab94359) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 3 : Human liver tissue lysate - total protein (ab29889)
    Lane 4 : Human brain tissue lysate - total protein (ab29466)
    Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 6 : DU 145 (Human prostate carcinoma cell line) Whole Cell Lysate
    Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 51 kDa
    Observed band size : 47 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds    The predicted molecular weight of the IDH2 protein is 51-kDa. However, the protein sequence contains a 39 residue transit peptide at the N-terminus. This might explain the migration of the protein at a lower molecular weight than expected.
  • Immunocytochemistry/ Immunofluorescence - IDH2 antibody (ab94359)
    Immunocytochemistry/ Immunofluorescence - IDH2 antibody (ab94359)
    ICC/IF image of ab94359 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab94359, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) MCF7 cells at 1µg/ml, and in 4% PFA fixed (10 min) HepG2 cells at 1µg/ml.

References for Anti-IDH2 antibody (ab94359)

ab94359 has not yet been referenced specifically in any publications.

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