Overview

  • Product name
  • Description
    Rabbit polyclonal to IDH3A
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human IDH3A.

  • Positive control
    • This antibody gave a positive signal in Mouse and Rat Brain Tissue lysates as well as the following whole cell lysates: HeLa; Jurkat; A431; HepG2; Raji: PC12.

Properties

Applications

Our Abpromise guarantee covers the use of ab109686 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
ICC/IF Use a concentration of 1 µg/ml.

Target

  • Sequence similarities
    Belongs to the isocitrate and isopropylmalate dehydrogenases family.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • H IDH alpha antibody
    • HIDH alpha antibody
    • IDH3A antibody
    • IDH3A_HUMAN antibody
    • Isocitrate dehydrogenase (NAD+) alpha chain precursor antibody
    • Isocitrate dehydrogenase [NAD] subunit alpha antibody
    • Isocitrate dehydrogenase [NAD] subunit alpha mitochondrial antibody
    • Isocitrate dehydrogenase 3 (NAD+) alpha antibody
    • Isocitrate dehydrogenase antibody
    • Isocitric dehydrogenase antibody
    • Isocitric dehydrogenase subunit alpha antibody
    • mitochondrial antibody
    • NAD antibody
    • NAD(+)-specific ICDH subunit alpha antibody
    • NAD(H) specific isocitrate dehydrogenase alpha subunit precursor antibody
    • NAD+ specific ICDH antibody
    • NADsubunit alpha mitochondrial precursor antibody
    • OTTHUMP00000184906 antibody
    see all

Images

  • All lanes : Anti-IDH3A antibody (ab109686) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 5 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 6 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 7 : Brain (Mouse) Tissue Lysate
    Lane 8 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 39 kDa
    Observed band size : 39 kDa
    Additional bands at : 100 kDa,145 kDa,60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute
  • ICC/IF image of ab109686 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109686, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 1µg/ml, and in 4% formaldehyde fixed (10 min) HeLa and HepG2 cells at 1µg/ml.

References

ab109686 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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