Anti-IDH3A antibody (ab118278)
Key features and details
- Goat polyclonal to IDH3A
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IDH3A antibody
See all IDH3A primary antibodies -
Description
Goat polyclonal to IDH3A -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Dog, Pig -
Immunogen
Synthetic peptide:
DFTEEICRRVKDLD
, corresponding to C terminal amino acids 353-366 of Human IDH3A (NP_005521.1). -
Positive control
- Recombinant Human IDH3A protein (ab114559) can be used as a positive control in WB. Human Lymph Nodes and Daudi lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab118278 ia purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab118278 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 40 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 40 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Sequence similarities
Belongs to the isocitrate and isopropylmalate dehydrogenases family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 282446 Cow
- Entrez Gene: 479066 Dog
- Entrez Gene: 3419 Human
- Entrez Gene: 100157242 Pig
- Omim: 601149 Human
- SwissProt: P41563 Cow
- SwissProt: P54836 Dog
- SwissProt: P50213 Human
see all -
Alternative names
- H IDH alpha antibody
- HIDH alpha antibody
- IDH3A antibody
see all
Images
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Anti-IDH3A antibody (ab118278) at 0.1 µg/ml + Human Lymph Node lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 40 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Exposure time: 1 hour
Datasheets and documents
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Datasheet download
References (0)
ab118278 has not yet been referenced specifically in any publications.