• Product nameAnti-IDH3A antibody
    See all IDH3A primary antibodies
  • Description
    Rabbit polyclonal to IDH3A
  • SpecificityThis antibody reacts with IDH3A.
  • Tested applicationsSuitable for: ICC/IF, ELISA, WB, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    A region within synthetic peptide: IPGDGIGPEI SAAVMKIFDA AKAPIQWEER NVTAIQGPGG KWMIPSEAKE, corresponding to N terminal amino acids 37-86 of Human IDH3A

  • Positive control
    • Jurkat cell lysate and human skeletal muscle tissue


Our Abpromise guarantee covers the use of ab58641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
ELISA 1/312500.
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
IP Use at an assay dependent concentration.


Anti-IDH3A antibody images

  • Anti-IDH3A antibody (ab58641) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 40 kDa
    Observed band size : 40 kDa
  • All lanes : Anti-IDH3A antibody (ab58641) at 1/10000 dilution (For 16 hours at 4°C)

    Lane 1 : Mouse adipocyte whole cell lysate
    Lane 2 : Mouse adipocyte whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 40 kDa
    Observed band size : 40 kDa

    Exposure time : 1 second

    This image is courtesy of an anonymous Abreview

    Secondary antibody - anti-rabbit HRP (ab6721)

    See Abreview

  • IDH3A was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to IDH3A and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab58641.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 38kDa; IDH3A.
  • ICC/IF image of ab58641 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58641, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling IDH3A with ab58641 at 4µg/ml. Arrows indicate positively labelled skeletal muscle cells.

  • All lanes : Anti-IDH3A antibody (ab58641) at 2.5 µg/ml

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat cell lysate with blocking peptide at 2 µg/ml

    Lysates/proteins at 25 µg per lane.

    Predicted band size : 40 kDa

References for Anti-IDH3A antibody (ab58641)

This product has been referenced in:
  • Terasawa K  et al. Preferential recognition of isocitrate dehydrogenase by a rabbit monoclonal antibody (ab124797) against the C-terminal peptide of RANKL. J Immunol Methods N/A:N/A (2015). ICC/IF ; Mouse . Read more (PubMed: 25771969) »
  • Ward PS  et al. The potential for isocitrate dehydrogenase mutations to produce 2-hydroxyglutarate depends on allele specificity and subcellular compartmentalization. J Biol Chem 288:3804-15 (2013). WB ; Human . Read more (PubMed: 23264629) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (adipocyte)
Loading amount 10 µg
Specification adipocyte
Gel Running Conditions Reduced Denaturing (12% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Submitted Jun 11 2010