Anti-IDH3G antibody (ab118289)
Key features and details
- Goat polyclonal to IDH3G
- Suitable for: WB
- Reacts with: Rat, Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-IDH3G antibody
See all IDH3G primary antibodies -
Description
Goat polyclonal to IDH3G -
Host species
Goat -
Specificity
ab118289 is expected to recognize both reported isoforms of IDH3G (NP_004126.1; NP_777358.1). -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat, Human, Pig
Predicted to work with: Cow, Dog -
Immunogen
Synthetic peptide:
DHLKLHSYATSIRK
with a Cysteine residue linker, corresponding to internal sequence amino acids 337-350 of Human IDH3G (NP_004126.1; NP_777358.1). -
Positive control
- MCF7 lysate; Human, Mouse and Rat heart lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab118289 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab118289 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 43 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 43 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Sequence similarities
Belongs to the isocitrate and isopropylmalate dehydrogenases family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 614145 Cow
- Entrez Gene: 481081 Dog
- Entrez Gene: 3421 Human
- Entrez Gene: 25179 Rat
- Omim: 300089 Human
- SwissProt: IDH3G Cow
- SwissProt: P51553 Human
- SwissProt: IDH3G Pig
see all -
Alternative names
- H IDHG antibody
- IDH gamma antibody
- IDH3G antibody
see all
Images
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All lanes : Anti-IDH3G antibody (ab118289) at 1 µg/ml
Lane 1 : Rat heart lysate
Lane 2 : Pig heart lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 43 kDa -
Anti-IDH3G antibody (ab118289) at 0.5 µg/ml + MCF7 lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 43 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (0)
ab118289 has not yet been referenced specifically in any publications.