Overview

  • Product name
  • Description
    Goat polyclonal to IDH3G
  • Specificity
    ab118289 is expected to recognize both reported isoforms of IDH3G (NP_004126.1; NP_777358.1).
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
    Predicted to work with: Cow, Dog
  • Immunogen

    Synthetic peptide:

    DHLKLHSYATSIRK

    with a Cysteine residue linker, corresponding to internal sequence amino acids 337-350 of Human IDH3G (NP_004126.1; NP_777358.1).

  • Positive control
    • MCF7 lysate; Human, Mouse and Rat heart lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab118289 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 43 kDa).

Target

Images

  • All lanes : Anti-IDH3G antibody (ab118289) at 1 µg/ml

    Lane 1 : Rat heart lysate
    Lane 2 : Pig heart lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 43 kDa
  • Anti-IDH3G antibody (ab118289) at 0.5 µg/ml + MCF7 lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 43 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)

References

ab118289 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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