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Rabbit polyclonal to IDI1
HumanPredicted to work with:
Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
Synthetic peptide, corresponding to a region within internal sequence amino acids 144-193 (SHPLSNPAEL EESDALGVRR AAQRRLKAEL GIPLEEVPPE EINYLTRIHY) of human IDI1 (NP_004499).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
Purified by peptide affinity chromatography method.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use at an assay dependent concentration.
ELISA titre using peptide based assay, 1:1562500.
Catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its highly electrophilic allylic isomer, dimethylallyl diphosphate (DMAPP).
Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from isopentenyl diphosphate: step 1/1.
Belongs to the IPP isomerase type 1 family.
Contains 1 nudix hydrolase domain.
Information by UniProt
- isopentenyl diphosphate dimethylallyl diphosphate isomerase antibody
- IDI1 antibody
- IDI1_HUMAN antibody
Western blot - IDI1 antibody (ab81491)
Anti-IDI1 antibody (ab81491) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µgSecondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size:
26 kDaObserved band size:
32 kDa (why is the actual band size different from the predicted?
)Additional bands at:
68 kDa. We are unsure as to the identity of these extra bands.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"