Overview

  • Product name
  • Description
    Rabbit polyclonal to IDI1
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 12-200 of Human IDI1 (NP_004499; UniProt ID: Q13907 isoform 2).

  • Positive control
    • WB: Huh7, Meningioma, MOLT4 cell lines IHC-P: SCC15 xenograft IF/ICC: HepG2 cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab97448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 32 kDa.
IHC-P 1/100.
ICC/IF Use a concentration of 5 µg/ml.

Target

Images

  • ab97448 stained HepG2 cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97448 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-IDI1 antibody (ab97448) at 1/3000 dilution + MOLT4 whole cell lysate at 30 µg

    Predicted band size : 32 kDa
    12% SDS PAGE
  • Immunohistochemical analysis of IDI1 in paraffin embedded SCC15 xenograft, using ab97448 at a 1/100 dilution.

References

ab97448 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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