Overview

  • Product name
    Anti-IDN3 antibody [KT55]
    See all IDN3 primary antibodies
  • Description
    Rat monoclonal [KT55] to IDN3
  • Specificity
    Recognises Human and Mouse IDN3 isoform B (short form).
  • Tested applications
    Suitable for: WB, IP, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Human IDN3 peptide coupled to a carrier protein(C terminal).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Purified from culture supernatant using a protein G column.
  • Clonality
    Monoclonal
  • Clone number
    KT55
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab106768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 304 kDa.
IP Use a concentration of 2 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Probably plays a structural role in chromatin. Involved in sister chromatid cohesion, possibly by interacting with the cohesin complex.
  • Tissue specificity
    Widely expressed. Highly expressed in heart, skeletal muscle, fetal and adult liver, fetal and adult kidney. Expressed at intermediates level in thymus, placenta, peripheral leukocyte and small intestine. Weakly or not expressed in brain, colon, spleen and lung.
  • Involvement in disease
    Cornelia de Lange syndrome 1
  • Sequence similarities
    Belongs to the SCC2/Nipped-B family.
    Contains 5 HEAT repeats.
  • Developmental stage
    In embryos, it is expressed in developing limbs and later in cartilage primordia of the ulna and of various hand bones. Sites of craniofacial expression include the cartilage primordium of the basioccipital and basisphenoid skull bones and elsewhere in the head and face, including a region encompassing the mesenchyme adjacent to the cochlear canal. Also expressed in the spinal column, notochord and surface ectoderm sclerotome and what seem to be migrating myoblasts. Expressed in the developing heart in the atrial and ventricular myocardium and in the ventricular tubeculae but absent in the endocardial cushions. Also expressed in the developing esophagus, trachea and midgut loops, in the bronchi of the lung and in the tubules of the metanephros. Expression in organs and tissues not typically affected in CDL (e.g. the developing trachea, bronchi, esophagus, heart and kidney) may reflect a bias towards underreporting of more subtle aspects of the phenotype or problems that typically present later in life. Expressed in the mesenchyme surrounding the cochlear canal possibly reflecting the hearing impairment commonly found. Weakly or not expressed in embryonic brain.
  • Domain
    Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Form
    There are 3 isoforms produced by alternative splicing. Isoform 1 also known as: A; IDN3-A. Isoform 2 also known as: B; IDN3-B.
  • Alternative names
    • CDLS antibody
    • Colon tumor susceptibility 2 antibody
    • Delangin antibody
    • DKFZp434L1319 antibody
    • FLJ11203 antibody
    • FLJ12597 antibody
    • FLJ13354 antibody
    • FLJ13648 antibody
    • FLJ44854 antibody
    • IDN 3 antibody
    • IDN 3 protein antibody
    • IDN 3 protein isoform A antibody
    • IDN 3 protein isoform B antibody
    • IDN 3B antibody
    • IDN3 B antibody
    • IDN3 protein antibody
    • IDN3 protein isoform A antibody
    • IDN3 protein isoform B antibody
    • IDN3B antibody
    • Mis 4 antibody
    • Mis4 antibody
    • Nipbl antibody
    • NIPBL_HUMAN antibody
    • Nipped B homolog (Drosophila) antibody
    • Nipped B homolog antibody
    • Nipped B like antibody
    • Nipped B like protein antibody
    • Nipped-B-like protein antibody
    • Scc 2 antibody
    • SCC 2 homolog antibody
    • Scc2 antibody
    • SCC2 homolog antibody
    • Sister chromatid cohesion protein Mis4 antibody
    see all

Anti-IDN3 antibody [KT55] images

  • Overlay histogram showing HepG2 cells stained with ab106768 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab106768, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rat IgG (H+L) (ab150165) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (ab18450, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • All lanes : Anti-IDN3 antibody [KT55] (ab106768) at 1/2000 dilution

    Lane 1 : Spectra Multicolor High Range Protein Marker
    Lane 2 : HT1080 cell lysate

    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 304 kDa
    Observed band size : 315 kDa (why is the actual band size different from the predicted?)


    Exposure time : 15 minutes

    This image was taken from an abreview submitted by Leisha Nolen.

    This western blot was run using a Tris-Acetate NuPage 3-8% Gel. For more information please see abreview.

    See Abreview

References for Anti-IDN3 antibody [KT55] (ab106768)

This product has been referenced in:
  • Nolen LD  et al. Regional chromatin decompaction in Cornelia de Lange syndrome associated with NIPBL disruption can be uncoupled from cohesin and CTCF. Hum Mol Genet 22:4180-93 (2013). WB ; Human . Read more (PubMed: 23760082) »

See 1 Publication for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% TritonX100 in PBS
Specification
HeLa
Fixative
Paraformaldehyde
Username

Dr. Kirk Mcmanus

Verified customer

Submitted Aug 07 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Purified protein (HT1080)
Loading amount
10000 cells
Specification
HT1080
Gel Running Conditions
Reduced Denaturing (Tris-Acetate NuPage Gel 3-8%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Dr. Leisha Nolen

Verified customer

Submitted Feb 03 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
ChIP
Sample
Human Cell lysate - nuclear (HT1080)
Specification
HT1080
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Detection step
Real-time PCR
Positive control
H3K9 trimethylation antibody. The Actin promoter and multiple genes that are known to be bound by NIPBL in other organisms.
Negative control
Multiple sites that are in gene poor regions and are not thought to bind based on studies in other species.
Username

Dr. Leisha Nolen

Verified customer

Submitted Dec 05 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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