Anti-IdU antibody [OTI2F8] (ab187742)
Key features and details
- Rat monoclonal [OTI2F8] to IdU
- Suitable for: IHC-P
- Isotype: IgG2a
Overview
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Product name
Anti-IdU antibody [OTI2F8] -
Description
Rat monoclonal [OTI2F8] to IdU -
Host species
Rat -
Specificity
ab187742 has been shown to cross react with BrdU. Cross-reactivity with other similar molecules has not been tested.
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Tested applications
Suitable for: IHC-Pmore details -
Immunogen
Chemical/ Small Molecule corresponding to IdU conjugated to Keyhole Limpet Haemocyanin (KLH).
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Positive control
- Colon tissue from IdU injected mouse.
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General notes
The clone number has been updated from 2F8 to OTI2F8, both clone numbers name the same clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 50% Glycerol, 1% BSA -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
ab187742 is purified from cell culture supernatant by affinity chromatography. -
Clonality
Monoclonal -
Clone number
OTI2F8 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab187742 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/150.
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Notes |
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IHC-P
1/150. |
Target
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Relevance
Iododeoxyuridine (5-Iodo-2'-deoxyuridine, IdU) is a synthetic thymidine nucleoside analog. IdU, like BrdU thymidine analog, is used to allow the detection of growing or proliferating cells in living tissues. During the S-phase of cell division, DNA replication occurs, and IdU can be incorporated into the newly synthesized DNA by substituting for naturally occurring thymidine. Antibodies specific for IdU are subsequently used to detect the incorporated IdU thymidine analog. This highlights cells that were actively replicating their DNA and is suggestive of actively growing cells. Antibody binding usually requires the DNA to be denatured, typically by exposing the cells to acid or heat. -
Alternative names
- 5-Iodo-2'-deoxyuridine antibody
- Iododeoxyuridine antibody
Images
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab187742 has been referenced in 2 publications.
- Azzoni V et al. BMI1 nuclear location is critical for RAD51-dependent response to replication stress and drives chemoresistance in breast cancer stem cells. Cell Death Dis 13:96 (2022). PubMed: 35110528
- Smith R et al. Poly(ADP-ribose)-dependent chromatin unfolding facilitates the association of DNA-binding proteins with DNA at sites of damage. Nucleic Acids Res 47:11250-11267 (2019). PubMed: 31566235