Overview

  • Product name
    Anti-IdU antibody [OTI2F8]
  • Description
    Rat monoclonal [OTI2F8] to IdU
  • Host species
    Rat
  • Tested applications
    Suitable for: IHC-Pmore details
  • Immunogen

    Chemical/ Small Molecule corresponding to IdU conjugated to Keyhole Limpet Haemocyanin (KLH).

  • Positive control
    • Colon tissue from IdU injected mouse.
  • General notes

    The clone number has been updated from 2F8 to OTI2F8, both clone numbers name the same clone.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 48% PBS, 50% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    ab187742 is purified from cell culture supernatant by affinity chromatography.
  • Clonality
    Monoclonal
  • Clone number
    OTI2F8
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab187742 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/150.

Target

  • Relevance
    Iododeoxyuridine (5-Iodo-2'-deoxyuridine, IdU) is a synthetic thymidine nucleoside analog. IdU, like BrdU thymidine analog, is used to allow the detection of growing or proliferating cells in living tissues. During the S-phase of cell division, DNA replication occurs, and IdU can be incorporated into the newly synthesized DNA by substituting for naturally occurring thymidine. Antibodies specific for IdU are subsequently used to detect the incorporated IdU thymidine analog. This highlights cells that were actively replicating their DNA and is suggestive of actively growing cells. Antibody binding usually requires the DNA to be denatured, typically by exposing the cells to acid or heat.
  • Alternative names
    • 5-Iodo-2'-deoxyuridine antibody
    • Iododeoxyuridine antibody

Images

  • Immunohistochemical analysis of paraffin-embedded colon tissue from IdU injected mouse, labeling IdU with ab187742 at 1/150 dilution.

  • Immunohistochemical analysis of paraffin-embedded colon tissue from BrdU injected mouse, labeling IdU with ab187742 at 1/150 dilution.

References

ab187742 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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