Anti-Iduronate 2 sulfatase antibody (ab189197)

Overview

  • Product name
    Anti-Iduronate 2 sulfatase antibody
    See all Iduronate 2 sulfatase primary antibodies
  • Description
    Goat polyclonal to Iduronate 2 sulfatase
  • Host species
    Goat
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Monkey, Gorilla
  • Immunogen

    Synthetic peptide corresponding to Human Iduronate 2 sulfatase aa 440-451 (internal sequence) (Cysteine residue). (NP_000193.1)
    Sequence:

    KHFRFRDLEEDP


    Database link: P22304

  • Positive control
    • Human placenta tissue: Human liver lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab189197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 62 kDa.
IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function
    Required for the lysosomal degradation of heparan sulfate and dermatan sulfate.
  • Tissue specificity
    Liver, kidney, lung, and placenta.
  • Involvement in disease
    Defects in IDS are the cause of mucopolysaccharidosis type 2 (MPS2) [MIM:309900]; also known as Hunter syndrome. MPS2 is an X-linked lysosomal storage disease characterized by intracellular accumulation of heparan sulfate and dermatan sulfate and their excretion in urine. Most children with MPS2 have a severe form with early somatic abnormalities including skeletal deformities, hepatosplenomegaly, and progressive cardiopulmonary deterioration. A prominent feature is neurological damage that presents as developmental delay and hyperactivity but progresses to mental retardation and dementia. They die before 15 years of age, usually as a result of obstructive airway disease or cardiac failure. In contrast, those with a mild form of MPS2 may survive into adulthood, with attenuated somatic complications and often without mental retardation.
  • Sequence similarities
    Belongs to the sulfatase family.
  • Post-translational
    modifications
    The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.
  • Cellular localization
    Lysosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha L iduronate sulfate sulfatase antibody
    • Alpha-L-iduronate sulfate sulfatase antibody
    • AW214631 antibody
    • Ids antibody
    • IDS_HUMAN antibody
    • Iduronate 2 sulfatase 14 kDa chain antibody
    • Iduronate 2 sulfatase 42 kDa chain antibody
    • Iduronate 2 sulfatase antibody
    • Iduronate 2-sulfatase 14 kDa chain antibody
    • Iduronate sulfatase antibody
    • Idursulfase antibody
    • MPS2 antibody
    • RP23-29M4.1 antibody
    • SIDS antibody
    see all

Images

  • Anti-Iduronate 2 sulfatase antibody (ab189197) at 0.1 µg/ml + Human Liver lysate in RIPA buffer at 35 µg/ml

    Developed using the ECL technique.

    Predicted band size: 62 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human placenta tissue labeling Iduronate 2 sulfatase with ab189197 at 3.75 µg/ml.

References

ab189197 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab189197.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up