Anti-Iduronate 2 sulfatase antibody (ab85701)

Overview

  • Product nameAnti-Iduronate 2 sulfatase antibody
    See all Iduronate 2 sulfatase primary antibodies
  • Description
    Goat polyclonal to Iduronate 2 sulfatase
  • SpecificityExpected to recognize isoform A (NP_000193.1).
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    KHFRFRDLEEDP

    by a Cysteine residue linker, corresponding to internal sequence amino acids 440-451 of Human Iduronate 2 sulfatase (NP_000193.1)

  • Positive control
    • Human Liver lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab85701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).

Target

  • FunctionRequired for the lysosomal degradation of heparan sulfate and dermatan sulfate.
  • Tissue specificityLiver, kidney, lung, and placenta.
  • Involvement in diseaseDefects in IDS are the cause of mucopolysaccharidosis type 2 (MPS2) [MIM:309900]; also known as Hunter syndrome. MPS2 is an X-linked lysosomal storage disease characterized by intracellular accumulation of heparan sulfate and dermatan sulfate and their excretion in urine. Most children with MPS2 have a severe form with early somatic abnormalities including skeletal deformities, hepatosplenomegaly, and progressive cardiopulmonary deterioration. A prominent feature is neurological damage that presents as developmental delay and hyperactivity but progresses to mental retardation and dementia. They die before 15 years of age, usually as a result of obstructive airway disease or cardiac failure. In contrast, those with a mild form of MPS2 may survive into adulthood, with attenuated somatic complications and often without mental retardation.
  • Sequence similaritiesBelongs to the sulfatase family.
  • Post-translational
    modifications
    The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.
  • Cellular localizationLysosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha L iduronate sulfate sulfatase antibody
    • Alpha-L-iduronate sulfate sulfatase antibody
    • AW214631 antibody
    • Ids antibody
    • IDS_HUMAN antibody
    • Iduronate 2 sulfatase 14 kDa chain antibody
    • Iduronate 2 sulfatase 42 kDa chain antibody
    • Iduronate 2 sulfatase antibody
    • Iduronate 2-sulfatase 14 kDa chain antibody
    • Iduronate sulfatase antibody
    • Idursulfase antibody
    • MPS2 antibody
    • RP23-29M4.1 antibody
    • SIDS antibody
    see all

Anti-Iduronate 2 sulfatase antibody images

  • Anti-Iduronate 2 sulfatase antibody (ab85701) at 0.1 µg/ml + Human Liver lysate at 35 µg

    Predicted band size : 62 kDa
    Observed band size : 62 kDa
  • ab85701 (3.8µg/ml) staining of paraffin embedded Human Placenta shows lysosomal staining of trophoblasts. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

References for Anti-Iduronate 2 sulfatase antibody (ab85701)

ab85701 has not yet been referenced specifically in any publications.

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