Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab59133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 34 kDa.

Target

  • RelevanceIER5 is a similar protein to other immediate early response proteins. In mouse, a similar protein may play an important role in mediating the cellular response to mitogenic signals.
  • Database links
  • Alternative names
    • Immediate early response 5 antibody
    • MGC102760 antibody
    • SBBI48 antibody

Anti-IER5 antibody images

  • All lanes : Anti-IER5 antibody (ab59133)

    Lane 1 : Human H1299 cell lysate transfected with empty vector
    Lane 2 : Human H1299 cells lysate overexpressing IER5

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP conjugated rabbit polyclonal to goat IgG at 1/1000 dilution

    Predicted band size : 34 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-IER5 antibody (ab59133)

ab59133 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (H1299 cells overexpressing IER5)
Loading amount 10 µg
Specification H1299 cells overexpressing IER5
Gel Running Conditions Reduced Denaturing (10 %)
Blocking step Blocking One (Nakalai) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 10 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"