Overview

  • Product name
    Anti-IER5 antibody
  • Description
    Goat polyclonal to IER5
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity

    Predicted to work with: Human
  • Immunogen

    Synthetic peptide:

    C-QPPSGGEDDDAEE

    , corresponding to internal sequence amino acids 248-260 of Human IER5

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab59133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 34 kDa.

Target

  • Relevance
    IER5 is a similar protein to other immediate early response proteins. In mouse, a similar protein may play an important role in mediating the cellular response to mitogenic signals.
  • Database links
  • Alternative names
    • Immediate early response 5 antibody
    • MGC102760 antibody
    • SBBI48 antibody

Images

  • All lanes : Anti-IER5 antibody (ab59133)

    Lane 1 : Human H1299 cell lysate transfected with empty vector
    Lane 2 : Human H1299 cells lysate overexpressing IER5

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP conjugated rabbit polyclonal to goat IgG at 1/1000 dilution

    Predicted band size : 34 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an anonymous Abreview

    See Abreview

References

ab59133 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (H1299 cells overexpressing IER5)
Loading amount
10 µg
Specification
H1299 cells overexpressing IER5
Gel Running Conditions
Reduced Denaturing (10 %)
Blocking step
Blocking One (Nakalai) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 10 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up