The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 34 kDa. Optimal concentration of antibody depends the protein level in a given sample. Our recommended concentrations are the starting point for general use. Customers are advised have to do a series of titration tests to determine optimal concentration. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
RelevanceIER5 is a similar protein to other immediate early response proteins. In mouse, a similar protein may play an important role in mediating the cellular response to mitogenic signals.
Anti-IER5 antibody (ab97939) at 1 µg/ml + HepG2 cell lysate at 10 µg with 5% skim milk in PBS buffer
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 34 kDa
References for Anti-IER5 antibody (ab97939)
This product has been referenced in:
Robinson PM et al. Proteolytic processing of connective tissue growth factor in normal ocular tissues and during corneal wound healing. Invest Ophthalmol Vis Sci53:8093-103 (2012).
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