Overview

  • Product name
  • Description
    Rabbit polyclonal to IEX1
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to internal amino acids from Human IEX1.

  • Positive control
    • Adrenal tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab65152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 17 kDa.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function
    May play a role in the ERK signaling pathway by inhibiting the dephosphorylation of ERK by phosphatase PP2A-PPP2R5C holoenzyme. Acts also as an ERK downstream effector mediating survival. As a member of the NUPR1/RELB/IER3 survival pathway, may provide pancreatic ductal adenocarcinoma with remarkable resistance to cell stress, such as starvation or gemcitabine treatment.
  • Sequence similarities
    Belongs to the IER3 family.
  • Post-translational
    modifications
    Phosphorylated at Thr-18, Thr-123 and Ser-126 by MAPK1/ERK2 and probably MAPK3/ERK1. Upon phosphorylation by MAPK1/ERK2 and MAPK3/ERK1, acquires the ability to inhibit cell death induced by various stimuli.
    Glycosylated.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • DIF2 antibody
    • Differentiation-dependent gene 2 protein antibody
    • GLY96 antibody
    • IER3 antibody
    • IEX1_HUMAN antibody
    • IEX1L antibody
    • Immediate early protein GLY96 antibody
    • Immediate early response 3 antibody
    • Immediate early response 3 protein antibody
    • Immediately early gene X 1 antibody
    • PACAP-responsive gene 1 protein antibody
    • PRG 1 antibody
    • PRG1 antibody
    • Protein DIF 2 antibody
    • Protein DIF-2 antibody
    • Protein PRG1 antibody
    • Radiation-inducible immediate-early gene IEX-1 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human adrenal tissue, staining IEX1 with ab65152 at 5 µg/ml.

References

This product has been referenced in:
  • Shahid M  et al. IEX-1 deficiency induces browning of white adipose tissue and resists diet-induced obesity. Sci Rep 6:24135 (2016). IHC . Read more (PubMed: 27063893) »
  • Wang YP  et al. Identification of immediate early gene X-1 as a cellular target gene of hcmv-mir-UL148D. Int J Mol Med 31:959-66 (2013). Read more (PubMed: 23403649) »

See all 5 Publications for this product

Customer reviews and Q&As

In original this antibody was tested on many tissues using (automated staining): 1. heat induced antigen retrieval in pH 6.0 citrate buffer- 20minutes. 2. primary used for only 45 minutes RT Usually when automated staining is carried out the step...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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