Overview

  • Product nameAnti-IF-2(Mt) antibody
    See all IF-2(Mt) primary antibodies
  • Description
    Rabbit polyclonal to IF-2(Mt)
  • Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to amino acids 497-646 of Human IF-2(Mt)

  • Positive control
    • Human cell line A431, Human plasma lysate, Human nasopharynx tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab121243 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
ICC/IF Use a concentration of 1 - 4 µg/ml. The following is recommended: cell fixation with ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permealization with 0.1% Triton X-100 in PBS for 3x5 minutes.
WB 1/250 - 1/500.

Target

  • FunctionOne of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex.
  • Tissue specificityExpressed in all tissues examined. Highest level in skeletal muscle.
  • Sequence similaritiesBelongs to the IF-2 family.
  • Cellular localizationMitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • IF-2(Mt) antibody
    • IF-2Mt antibody
    • IF2(mt) antibody
    • IF2M_HUMAN antibody
    • mitochondrial antibody
    • mitochondrial translational initiation factor 2 antibody
    • Mtif2 antibody
    • Translation initiation factor IF-2 antibody
    • Translation initiation factor IF-2, mitochondrial antibody
    see all

Anti-IF-2(Mt) antibody images

  • All lanes : Anti-IF-2(Mt) antibody (ab121243) at 1/250 dilution

    Lane 1 : Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
    Lane 2 : RT-4 cell lysate
    Lane 3 : U-251 MG cell lysate
    Lane 4 : Human Plasma lysate
    Lane 5 : Human liver lysate
    Lane 6 : Human tonsil lysate
  • Immunohistochemical staining of paraffin embedded Human nasopharynx tissue, using 1/25 ab121243, shows strong membranous positivity in respiratory epithelial cells.
  • Immunofluorescent staining, using 4 µg/ml ab121243, in Human cell line A431 shows positivity in mitochondria. Antibody staining is shown in green.

References for Anti-IF-2(Mt) antibody (ab121243)

ab121243 has not yet been referenced specifically in any publications.

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