• Product nameAnti-IF3EI antibody
    See all IF3EI primary antibodies
  • Description
    Rabbit polyclonal to IF3EI
  • Tested applicationsSuitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from N-terminal of human IF3EI.

  • Positive control
    • HUVEC cells


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab64900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.
WB 1/500 - 1/1000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).


  • FunctionComponent of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
  • Sequence similaritiesBelongs to the eIF-3 subunit L family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • eIEF associated protein HSPC021 antibody
    • EIF3EIP antibody
    • eif3l antibody
    • EIF3L_HUMAN antibody
    • EIF3S11 antibody
    • EIF3S6IP antibody
    • Eukaryotic translation initiation factor 3 subunit 6 interacting antibody
    • Eukaryotic translation initiation factor 3 subunit 6-interacting protein antibody
    • Eukaryotic translation initiation factor 3 subunit E-interacting protein antibody
    • Eukaryotic translation initiation factor 3 subunit L antibody
    • eukaryotic translation initiation factor 3, subunit E interacting antibody
    • HSPC021 antibody
    • HSPC025 antibody
    • MSTP005 antibody
    see all

Anti-IF3EI antibody images

  • All lanes : Anti-IF3EI antibody (ab64900) at 1/500 dilution

    Lane 1 : extracts from HUVEC cells
    Lane 2 : extracts from HUVEC cells with immunizing peptide at 10 µg

    Lysates/proteins at 10 µg per lane.

    Predicted band size : 67 kDa
    Observed band size : 67 kDa
  • IHC-P image of IF3EI antibody (ab64900) on pancreas tissue sections. The sections were fixed in Paraformaldehyde and underwent heat mediated antigen retrieval using Novacastra (pH6). The sections were then blocked in 3% H2O2 solution for 10 minutes at 22°C.

    See Abreview

References for Anti-IF3EI antibody (ab64900)

ab64900 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample African Green Monkey Cell lysate - whole cell (Vero cells)
Loading amount 120000 cells
Specification Vero cells
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jun 27 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Pancreas)
Specification Pancreas
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Novocastra pH 6 retrieval buffer
Permeabilization No
Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 22°C

Dr. Aamir Ahmed

Verified customer

Submitted Jan 25 2011