Overview

  • Product name
  • Description
    Rabbit polyclonal to IFI16
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide corresponding to a region within internal amino acids 101-150 (EAGPATPTST TSHMLASERG ETSATQEETS TAQAGTSTAQ ARTSTAQAGT) of Mouse IFI16 (NP_032355).

  • Positive control
    • Mouse lung lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab104409 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 69 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Relevance
    Interferon (IFN) Inducible 16 (IFI16) protein belongs to a family of HIN 200 human and mouse proteins. IFI16 is a nuclear protein comtaining regulatory domains such as DNA binding domain, transcriptional regulatory domain and DAPIN/PAAD domain. IFI16 has three isotypes A, B, and C (85-95 kDa), which arise as a result of mRNA alternative splicing. All are phosphorylated on serine and threonine residues and can homo and heterodimerize. Expression is restricted to the nuclei of hematopoietic cells, fibroblasts and epithelial cells. IFI16 expression in hematopoietic cells of myeloid lineage is tightly regulated and highly induced in the differentiation and proliferation of the cell. Due to its localization in the nucleus, regulation of protein expression, and ability to bind DNA, it is assumed that IFI16 has a role in transcription regulation of cell differentiation. In addition, it was found that IFI16 can act as a transcriptional repressor and is involved in regulation and activation of p53 in cancer cells.
  • Cellular localization
    Nucleus. Cytoplasm. Note: Cellular distribution is dependent on the acetylation status of the multipartite nuclear localization signal (NLS); NLS acetylation promotes cytoplasmic localization.
  • Database links
  • Alternative names
    • IFI16 antibody
    • Gamma interferon inducible protein 16 antibody
    • Gamma-interferon-inducible protein Ifi16 antibody
    • IF16_HUMAN antibody
    • Ifi204 antibody
    • IFNGIP1 antibody
    • interferon activated gene 204 antibody
    • Interferon gamma induced protein IFI 16 antibody
    • Interferon gamma inducible protein 16 antibody
    • Interferon-inducible myeloid differentiation transcriptional activator antibody
    • p204 antibody
    • PYHIN2 antibody
    see all

Images

  • Anti-IFI16 antibody (ab104409) at 1 µg/ml + Mouse lung lysate at 10 µg

    Predicted band size : 69 kDa

References

This product has been referenced in:
  • Zhu W  et al. p204-initiated innate antiviral response in mouse Leydig cells. Biol Reprod 91:8 (2014). Mouse . Read more (PubMed: 24876405) »
  • Khare S  et al. The PYRIN domain-only protein POP3 inhibits ALR inflammasomes and regulates responses to infection with DNA viruses. Nat Immunol 15:343-53 (2014). Read more (PubMed: 24531343) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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