Overview

  • Product name
  • Description
    Rabbit polyclonal to IFI16
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human IFI16.
    Database link: Q16666

  • Positive control
    • Mouse spleen and lung extracts

Properties

Applications

Our Abpromise guarantee covers the use of ab185812 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 88 kDa.
IHC-P 1/50 - 1/200.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance
    Interferon (IFN) Inducible 16 (IFI16) protein belongs to a family of HIN 200 human and mouse proteins. IFI16 is a nuclear protein comtaining regulatory domains such as DNA binding domain, transcriptional regulatory domain and DAPIN/PAAD domain. IFI16 has three isotypes A, B, and C (85-95 kDa), which arise as a result of mRNA alternative splicing. All are phosphorylated on serine and threonine residues and can homo and heterodimerize. Expression is restricted to the nuclei of hematopoietic cells, fibroblasts and epithelial cells. IFI16 expression in hematopoietic cells of myeloid lineage is tightly regulated and highly induced in the differentiation and proliferation of the cell. Due to its localization in the nucleus, regulation of protein expression, and ability to bind DNA, it is assumed that IFI16 has a role in transcription regulation of cell differentiation. In addition, it was found that IFI16 can act as a transcriptional repressor and is involved in regulation and activation of p53 in cancer cells.
  • Cellular localization
    Nucleus. Cytoplasm. Note: Cellular distribution is dependent on the acetylation status of the multipartite nuclear localization signal (NLS); NLS acetylation promotes cytoplasmic localization.
  • Database links
  • Alternative names
    • IFI16 antibody
    • Gamma interferon inducible protein 16 antibody
    • Gamma-interferon-inducible protein Ifi16 antibody
    • IF16_HUMAN antibody
    • Ifi204 antibody
    • IFNGIP1 antibody
    • interferon activated gene 204 antibody
    • Interferon gamma induced protein IFI 16 antibody
    • Interferon gamma inducible protein 16 antibody
    • Interferon-inducible myeloid differentiation transcriptional activator antibody
    • p204 antibody
    • PYHIN2 antibody
    see all

Images

  • Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab185812. Blue DAPI for nuclear staining.
  • All lanes : Anti-IFI16 antibody (ab185812) at 1/500 dilution

    Lane 1 : Mouse spleen extract
    Lane 2 : Mouse lung extract

    Predicted band size: 88 kDa

References

ab185812 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Endothelial Cells)
Gel Running Conditions
Reduced Denaturing (7.5 % SDS PAGE)
Loading amount
30 µg
Specification
Endothelial Cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

Submitted Dec 05 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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