Overview

  • Product nameAnti-IFI27 antibody
    See all IFI27 primary antibodies
  • Description
    Rabbit polyclonal to IFI27
  • Tested applicationsSuitable for: ELISA, WB, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: multiple antigenic peptide (8 branch) with the sequence

    EASALTSSAVTS

    , corresponding to amino acids 2-13 of Human IFI27

  • Positive control
    • SHSY5Y neuroblastoma cells

Properties

Applications

Our Abpromise guarantee covers the use of ab14695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000 - 1/100000.
WB Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionPromotes cell death. Mediates IFN-induced apoptosis characterized by a rapid and robust release of cytochrome C from the mitochondria and activation of BAX and caspases 2, 3, 6, 8 and 9.
  • Sequence similaritiesBelongs to the IFI6/IFI27 family.
  • Cellular localizationMitochondrion. Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2310061N23Rik antibody
    • FAM 14D antibody
    • FAM14D antibody
    • IFI 27 antibody
    • IFI27 antibody
    • IFI27_HUMAN antibody
    • Interferon alpha induced 11.5 kDa protein antibody
    • Interferon alpha inducible protein 27 antibody
    • Interferon alpha-induced 11.5 kDa protein antibody
    • Interferon alpha-inducible protein 27 antibody
    • interferon alpha-inducible protein 27, mitochondrial antibody
    • Interferon inducible protein 27 antibody
    • interferon-stimulated gene 12 antibody
    • Interferon-stimulated gene 12a protein antibody
    • ISG 12 antibody
    • ISG12 antibody
    • ISG12(a) antibody
    • ISG12(a) protein antibody
    • ISG12A antibody
    • mitochondrial antibody
    • p27 antibody
    see all

Anti-IFI27 antibody images

  • ICC/IF image of ab14695 stained SKNSH cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14695, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-IFI27 antibody (ab14695)

This product has been referenced in:
  • Li S  et al. Interferon alpha-inducible protein 27 promotes epithelial-mesenchymal transition and induces ovarian tumorigenicity and stemness. J Surg Res 193:255-64 (2015). Read more (PubMed: 25103640) »
  • Yang D  et al. MiR-942 Mediates Hepatitis C Virus-Induced Apoptosis via Regulation of ISG12a. PLoS One 9:e94501 (2014). WB ; Human . Read more (PubMed: 24727952) »

See all 2 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Chimpanzee Tissue sections (Lymph Node)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9
Permeabilization No
Specification Lymph Node
Fixative Formaldehyde
Username

Dr. Edward Greenwood

Verified customer

Submitted Jan 25 2016

resolved with credit note

Application Western blot
Sample Human Cell lysate - whole cell (293T)
Specification 293T
Gel Running Conditions Reduced Denaturing
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 04 2007

Thank you for your enquiry. Ab14695 was raised against a multiple antigenic peptide (8 branch) with the following sequence: N - EASALTSSAVTS - 'C I have updated the datasheet to reflect this information for future reference. I hope this infor...

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Thank you for your enquiry and your patience in awaiting a response. The antibody was tested on thin frozen tissue sections as well as several cell lines including SHSY5Y neuroblastoma cells. If you have any further questions then please do not hesitat...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"