- Datasheet
- Specific References (2)
- Protocols
Overview
-
Product nameAnti-IFI27 antibody
See all IFI27 primary antibodies -
DescriptionRabbit polyclonal to IFI27
-
Host speciesRabbit
-
Tested applicationsSuitable for: ELISA, WB, IHC-Fr, ICC/IFmore details
-
Species reactivityReacts with: Human
-
Immunogen
Synthetic peptide: multiple antigenic peptide (8 branch) with the sequence
EASALTSSAVTS
, corresponding to amino acids 2-13 of Human IFI27 -
Positive control
- SHSY5Y neuroblastoma cells
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
-
Concentration information loading... -
PurityImmunogen affinity purified
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab14695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | 1/10000 - 1/100000. | |
| WB | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| ICC/IF | Use a concentration of 5 µg/ml. |
Target
-
FunctionPromotes cell death. Mediates IFN-induced apoptosis characterized by a rapid and robust release of cytochrome C from the mitochondria and activation of BAX and caspases 2, 3, 6, 8 and 9.
-
Sequence similaritiesBelongs to the IFI6/IFI27 family.
-
Cellular localizationMitochondrion. Membrane.
- Information by UniProt
-
Database links
- Entrez Gene: 3429 Human
- Omim: 600009 Human
- SwissProt: P40305 Human
- Unigene: 532634 Human
-
Alternative names
- 2310061N23Rik antibody
- FAM 14D antibody
- FAM14D antibody
see all
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-IFI27 antibody (ab14695)ICC/IF image of ab14695 stained SKNSH cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14695, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
References
This product has been referenced in:
- Li S et al. Interferon alpha-inducible protein 27 promotes epithelial-mesenchymal transition and induces ovarian tumorigenicity and stemness. J Surg Res 193:255-64 (2015). Read more (PubMed: 25103640) »
- Yang D et al. MiR-942 Mediates Hepatitis C Virus-Induced Apoptosis via Regulation of ISG12a. PLoS One 9:e94501 (2014). WB ; Human . Read more (PubMed: 24727952) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"