Customer reviews and FAQs

Overview

  • Product name
    Anti-IFI27 antibody
    See all IFI27 primary antibodies
  • Description
    Rabbit polyclonal to IFI27
  • Tested applications
    Suitable for: ELISA, WB, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: multiple antigenic peptide (8 branch) with the sequence

    EASALTSSAVTS

    , corresponding to amino acids 2-13 of Human IFI27

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • SHSY5Y neuroblastoma cells

Properties

Applications

Our Abpromise guarantee covers the use of ab14695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000 - 1/100000.
WB Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    Promotes cell death. Mediates IFN-induced apoptosis characterized by a rapid and robust release of cytochrome C from the mitochondria and activation of BAX and caspases 2, 3, 6, 8 and 9.
  • Sequence similarities
    Belongs to the IFI6/IFI27 family.
  • Cellular localization
    Mitochondrion. Membrane.
  • Information by UniProt
  • Database links
    • Alternative names
      • 2310061N23Rik antibody
      • FAM 14D antibody
      • FAM14D antibody
      • IFI 27 antibody
      • IFI27 antibody
      • IFI27_HUMAN antibody
      • Interferon alpha induced 11.5 kDa protein antibody
      • Interferon alpha inducible protein 27 antibody
      • Interferon alpha-induced 11.5 kDa protein antibody
      • Interferon alpha-inducible protein 27 antibody
      • interferon alpha-inducible protein 27, mitochondrial antibody
      • Interferon inducible protein 27 antibody
      • interferon-stimulated gene 12 antibody
      • Interferon-stimulated gene 12a protein antibody
      • ISG 12 antibody
      • ISG12 antibody
      • ISG12(a) antibody
      • ISG12(a) protein antibody
      • ISG12A antibody
      • mitochondrial antibody
      • p27 antibody
      see all

    Anti-IFI27 antibody images

    • Immunocytochemistry/ Immunofluorescence - Anti-IFI27 antibody (ab14695)
      Immunocytochemistry/ Immunofluorescence - Anti-IFI27 antibody (ab14695)
      ICC/IF image of ab14695 stained SKNSH cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14695, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References for Anti-IFI27 antibody (ab14695)

    This product has been referenced in:
    • Li S  et al. Interferon alpha-inducible protein 27 promotes epithelial-mesenchymal transition and induces ovarian tumorigenicity and stemness. J Surg Res 193:255-64 (2015). Read more (PubMed: 25103640) »
    • Yang D  et al. MiR-942 Mediates Hepatitis C Virus-Induced Apoptosis via Regulation of ISG12a. PLoS One 9:e94501 (2014). WB ; Human . Read more (PubMed: 24727952) »

    See all 2 Publications for this product

    Publishing research using ab14695? Please let us know so that we can cite the reference in this datasheet.

    Product Wall

    Submit an abreview for ab14695 Submit a question for ab14695

    5 Abreviews or Q&A

    10
    Votes: Highest First

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IFI27 antibody

     Good
    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Chimpanzee Tissue sections (Lymph Node)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9
    Permeabilization
    No
    Specification
    Lymph Node
    Fixative
    Formaldehyde
    Read More
    abreview image
    Username

    Dr. Edward Greenwood

    Verified customer

    Submitted Jan 25 2016

    WB with human lung cell line
    sees no band, just background
    loaded 50 ug protein
    Ab: 1/500, 1/200
    block: 5% milk
    2nd Ab works well

    resolved with credit note

    Western blot abreview for Anti-IFI27 antibody

     Poor
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (293T)
    Specification
    293T
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More
    Username

    Abcam user community

    Verified customer

    Submitted Sep 04 2007

    I would like to know which peptide seqeunce is used for raising the IFI27 antibody Sincerely yours

    Thank you for your enquiry. Ab14695 was raised against a multiple antigenic peptide (8 branch) with the following sequence: N - EASALTSSAVTS - 'C I have updated the datasheet to reflect this information for future reference. I hope this infor...

    Read More

    I'd like to know what controls have been used with screening this antibody. Have you tested against recombinant protein, interferon-induced protein?

    Thank you for your enquiry and your patience in awaiting a response. The antibody was tested on thin frozen tissue sections as well as several cell lines including SHSY5Y neuroblastoma cells. If you have any further questions then please do not hesitat...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up