The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Titre using peptide based assay: 1/62500.
Use a concentration of 1 µg/ml. Predicted molecular weight: 12 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
RelevanceIFI27L2 (interferon, alpha-inducible protein 27-like 2) is also known as FAM14A but its exact function remains unknown. Like other ISG12 family members, it contains a conserved 80-amino acid ISG12 motif.
Cellular localizationMembrane; Single-pass type I membrane protein.