Anti-IFIT1 antibody [3G8] (ab118062)

Overview

  • Product nameAnti-IFIT1 antibody [3G8]
    See all IFIT1 primary antibodies
  • Description
    Mouse monoclonal [3G8] to IFIT1
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human IFIT1. Recombinant full length Human IFIT1 produced in HEK293T cells (NP_000899).
    Database link: P09914

  • Positive control
    • WB: HEK293T cell lysate transfected with pCMV6-ENTRY IFIT1 cDNA. IHC-P: Human liver carcinoma, Human ovary adenocarcinoma, kidney and liver tissues. ICC/IF: HT29 cells and COS7 cells transiently transfected by pCMV6-ENTRY IFIT1 cDNA. Flow: HEK293T cells transfected with pCMV6-ENTRY IFIT1 overexpress plasmid
  • General notesStable for 12 months from date of receipt.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 48% PBS, 1% BSA
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesab118062 is purified from Mouse ascites fluids by affinity chromatography.
  • ClonalityMonoclonal
  • Clone number3G8
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab118062 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 55 kDa.
IHC-P 1/50.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/50 - 1/100.

Target

  • Sequence similaritiesBelongs to the IFIT family.
    Contains 10 TPR repeats.
  • Information by UniProt
  • Database links
  • Alternative names
    • C56 antibody
    • G10P1 antibody
    • GARG 16 antibody
    • GARG16 antibody
    • IFI 56 antibody
    • IFI 56K antibody
    • IFI-56K antibody
    • IFI56 antibody
    • IFIT-1 antibody
    • Ifit1 antibody
    • IFIT1_HUMAN antibody
    • IFNAI1 antibody
    • Interferon alpha inducible protein (MW 56kD) antibody
    • Interferon induced 56 kDa protein antibody
    • Interferon induced protein 56 antibody
    • Interferon inducible mRNA 561 antibody
    • Interferon-induced 56 kDa protein antibody
    • Interferon-induced protein with tetratricopeptide repeats 1 antibody
    • ISG56 antibody
    • OTTHUMP00000020062 antibody
    • P56 antibody
    • RNM561 antibody
    see all

Anti-IFIT1 antibody [3G8] images

  • All lanes : Anti-IFIT1 antibody [3G8] (ab118062) at 1/500 dilution

    Lane 1 : HEK293T cell lyaste, transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cell lysate, transfected with pCMV6-ENTRY IFIT1 cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 55 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC203320)
  • ab118062 at 1/50 dilution staining IFIT1 in Paraffin-embedded Human Kidney tissue by Immunohistochemistry.
  • ab118062 at 1/50 dilution staining IFIT1 in Paraffin-embedded Human Liver tissue by Immunohistochemistry.
  • ab118062 at 1/50 dilution staining IFIT1 in Paraffin-embedded Human Liver carcinoma tissue by Immunohistochemistry.
  • ab118062 at 1/50 dilution staining IFIT1 in Paraffin-embedded Human Ovary adenocarcinoma tissue by Immunohistochemistry.
  • ab118062 at 1/50 dilution staining IFIT1 in COS7 cells transiently transfected by pCMV6-ENTRY IFIT1 by Immunofluorescence.
  • ab118062 at 1/50 dilution staining IFIT1 in HT29 cells by Immunofluorescence.
  • ab118062 at 1/100 dilution staining IFIT1 in HEK293T cells transfected with either pCMV6-ENTRY IFIT1 overexpress plasmid(Red) or empty vector control plasmid(Blue) by Flow Cytometry.

References for Anti-IFIT1 antibody [3G8] (ab118062)

ab118062 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"