Overview

  • Product nameAnti-IFNA4 antibody
    See all IFNA4 primary antibodies
  • Description
    Mouse polyclonal to IFNA4
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-189 of Human IFNA4

  • Positive control
    • Transfected 293Tcell lysate (WB) HeLa cells (ICC/IF)

Properties

Applications

Our Abpromise guarantee covers the use of ab67385 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notesICC/IF: Use at a concentration of 10 µg/ml. Purification with Protein A is recommended prior to proceeding with the ICC/IF protocol.

    WB: 1/500 - 1/1000. Detects a band of approximately 20 kDa (predicted molecular weight: 22 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceProduced by macrophages, IFN alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
    • Cellular localizationSecreted
    • Database links
    • Alternative names
      • IFN-alpha-4 antibody
      • IFN-alpha4a antibody
      • INFA4 antibody
      • Interferon alpha 4 precursor antibody
      • Interferon alpha 4B antibody
      • Interferon alpha 76 antibody
      • Interferon alpha M1 antibody
      • Interferon, alpha 4 antibody
      see all

    Anti-IFNA4 antibody images

    • ab67385, at a concentration 10 µg/ml, staining IFNA4 in HeLa cell by Immunofluorescence.
    • All lanes : Anti-IFNA4 antibody (ab67385) at 1/500 dilution

      Lane 1 : IFNA4 transfected 293T cell lysate
      Lane 2 : non transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/2500 dilution

      Predicted band size : 22 kDa
      Observed band size : 18,20 kDa (why is the actual band size different from the predicted?)

    References for Anti-IFNA4 antibody (ab67385)

    ab67385 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"