• Product nameAnti-IFNAR1 antibody [EP899Y]
    See all IFNAR1 primary antibodies
  • Description
    Rabbit monoclonal [EP899Y] to IFNAR1
  • SpecificityThis antibody is specific for the 90kda form and the full-length form of IFNAR1 protein.
  • Tested applicationsSuitable for: WB, Flow Cyt, IPmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues near the C-terminus of human IFNAR1.

  • Positive control
    • Permeabilised K562 cells, K562 cell lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.



Our Abpromise guarantee covers the use of ab45172 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 90,130 kDa (predicted molecular weight: 64 kDa). Due to extensive glycosylation of the mature molecule; the 90 kDa species is a minor incompletely processed species.
Flow Cyt 1/30. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP 1/50.
  • Application notesIs unsuitable for IHC-P.
  • Target

    • FunctionAssociates with IFNAR2 to form the type I interferon receptor. Receptor for interferons alpha and beta. Binding to type I IFNs triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and IFNR alpha- and beta-subunits themselves.
    • Tissue specificityIFN receptors are present in all tissues and even on the surface of most IFN-resistant cells. Isoform 1, isoform 2 and isoform 3 are expressed in the IFN-alpha sensitive myeloma cell line U266S. Isoform 2 and isoform 3 are expressed in the IFN-alpha resistant myeloma cell line U266R. Isoform 1 is not expressed in IFN-alpha resistant myeloma cell line U266R.
    • Sequence similaritiesBelongs to the type II cytokine receptor family.
      Contains 3 fibronectin type-III domains.
    • Post-translational
      Phosphorylated on tyrosine residues by TYK2 tyrosine kinase.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Alpha type antiviral protein antibody
      • AVP antibody
      • Beta type antiviral protein antibody
      • CRF2-1 antibody
      • Cytokine receptor class-II member 1 antibody
      • Cytokine receptor family 2 member 1 antibody
      • IFN alpha REC antibody
      • IFN alpha receptor antibody
      • IFN alpha/beta Receptor alpha antibody
      • IFN beta receptor antibody
      • IFN-alpha/beta receptor 1 antibody
      • IFN-R-1 antibody
      • IFNAR antibody
      • Ifnar1 antibody
      • IFNBR antibody
      • IFRC antibody
      • INAR1_HUMAN antibody
      • Interferon (alpha beta and omega) receptor 1 antibody
      • Interferon alpha/beta receptor 1 antibody
      • Interferon alpha/beta receptor alpha chain antibody
      • Interferon beta receptor 1 antibody
      • Type I interferon receptor 1 antibody
      see all

    Anti-IFNAR1 antibody [EP899Y] images

    • Anti-IFNAR1 antibody [EP899Y] (ab45172) at 1/20000 dilution + K562 cell lysate at 10 µg

      Goat anti rabbit HRP labelled. at 1/2000 dilution

      Predicted band size : 64 kDa
      Observed band size : 90,130 kDa (why is the actual band size different from the predicted?)
    • Flow cytometric analysis of permeabilized K562 cells using ab45172 at a 1/30 dilution (red) or a rabbit IgG (negative) (green).

    References for Anti-IFNAR1 antibody [EP899Y] (ab45172)

    This product has been referenced in:
    • Chandra PK  et al. Impaired expression of type I and type II interferon receptors in HCV-associated chronic liver disease and liver cirrhosis. PLoS One 9:e108616 (2014). Read more (PubMed: 25265476) »
    • Wan S  et al. Chemotherapeutics and radiation stimulate MHC class I expression through elevated interferon-beta signaling in breast cancer cells. PLoS One 7:e32542 (2012). WB ; Human . Read more (PubMed: 22396773) »

    See all 8 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Flow Cytometry
    Fixation BD #554722
    Sample Human Cell (primary human macrophages)
    Specification primary human macrophages
    Gating Strategy macrophages
    Permeabilization No

    Abcam user community

    Verified customer

    Submitted Jul 19 2013

    Thank you for contacting us.

    I would recommend you consult the manufacturer of the beads which you intent to use. The binding capacity will be determined by the manufacturer of the beads. For example Millipore'sPureProteome Protein A and P...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (vascular smooth muscle cells)
    Loading amount 10 µg
    Specification vascular smooth muscle cells
    Treatment DTT and 5 min boiling
    Gel Running Conditions Reduced Denaturing (4-12 % gradients)
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

    Abcam user community

    Verified customer

    Submitted Apr 27 2012

    Thank you for your enquiry. A general protocol for intracellular staining with this antibody can be found at the following link: http://www.abcam.com/ps/pdf/protocols/flow_intracellular_staining.pdf A second protocol describes indirect stainin...

    Read More