Overview

  • Product nameAnti-IFNAR1 antibody [EP899Y]
    See all IFNAR1 primary antibodies
  • Description
    Rabbit monoclonal [EP899Y] to IFNAR1
  • SpecificityThis antibody is specific for the 90kda form and the full-length form of IFNAR1 protein.
  • Tested applicationsSuitable for: WB, Flow Cyt, IPmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues near the C-terminus of human IFNAR1.

  • Positive control
    • Permeabilised K562 cells, K562 cell lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

Properties

Applications

Our Abpromise guarantee covers the use of ab45172 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 90,130 kDa (predicted molecular weight: 64 kDa). Due to extensive glycosylation of the mature molecule; the 90 kDa species is a minor incompletely processed species.
Flow Cyt 1/30. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP 1/50.
  • Application notesIs unsuitable for IHC-P.
  • Target

    • FunctionAssociates with IFNAR2 to form the type I interferon receptor. Receptor for interferons alpha and beta. Binding to type I IFNs triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and IFNR alpha- and beta-subunits themselves.
    • Tissue specificityIFN receptors are present in all tissues and even on the surface of most IFN-resistant cells. Isoform 1, isoform 2 and isoform 3 are expressed in the IFN-alpha sensitive myeloma cell line U266S. Isoform 2 and isoform 3 are expressed in the IFN-alpha resistant myeloma cell line U266R. Isoform 1 is not expressed in IFN-alpha resistant myeloma cell line U266R.
    • Sequence similaritiesBelongs to the type II cytokine receptor family.
      Contains 3 fibronectin type-III domains.
    • Post-translational
      modifications
      Phosphorylated on tyrosine residues by TYK2 tyrosine kinase.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Alpha type antiviral protein antibody
      • AVP antibody
      • Beta type antiviral protein antibody
      • CRF2-1 antibody
      • Cytokine receptor class-II member 1 antibody
      • Cytokine receptor family 2 member 1 antibody
      • IFN alpha REC antibody
      • IFN alpha receptor antibody
      • IFN alpha/beta Receptor alpha antibody
      • IFN beta receptor antibody
      • IFN-alpha/beta receptor 1 antibody
      • IFN-R-1 antibody
      • IFNAR antibody
      • Ifnar1 antibody
      • IFNBR antibody
      • IFRC antibody
      • INAR1_HUMAN antibody
      • Interferon (alpha beta and omega) receptor 1 antibody
      • Interferon alpha/beta receptor 1 antibody
      • Interferon alpha/beta receptor alpha chain antibody
      • Interferon beta receptor 1 antibody
      • Type I interferon receptor 1 antibody
      see all

    Anti-IFNAR1 antibody [EP899Y] images

    • Anti-IFNAR1 antibody [EP899Y] (ab45172) at 1/20000 dilution + K562 cell lysate at 10 µg

      Secondary
      Goat anti rabbit HRP labelled. at 1/2000 dilution

      Predicted band size : 64 kDa
      Observed band size : 90,130 kDa (why is the actual band size different from the predicted?)
    • Flow cytometric analysis of permeabilized K562 cells using ab45172 at a 1/30 dilution (red) or a rabbit IgG (negative) (green).

    References for Anti-IFNAR1 antibody [EP899Y] (ab45172)

    This product has been referenced in:
    • Chandra PK  et al. Impaired expression of type I and type II interferon receptors in HCV-associated chronic liver disease and liver cirrhosis. PLoS One 9:e108616 (2014). Read more (PubMed: 25265476) »
    • Wan S  et al. Chemotherapeutics and radiation stimulate MHC class I expression through elevated interferon-beta signaling in breast cancer cells. PLoS One 7:e32542 (2012). WB ; Human . Read more (PubMed: 22396773) »

    See all 8 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Flow Cytometry
    Fixation BD #554722
    Sample Human Cell (primary human macrophages)
    Specification primary human macrophages
    Gating Strategy macrophages
    Permeabilization No
    Username

    Abcam user community

    Verified customer

    Submitted Jul 19 2013

    Thank you for contacting us.

    I would recommend you consult the manufacturer of the beads which you intent to use. The binding capacity will be determined by the manufacturer of the beads. For example Millipore'sPureProteome Protein A and P...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (vascular smooth muscle cells)
    Loading amount 10 µg
    Specification vascular smooth muscle cells
    Treatment DTT and 5 min boiling
    Gel Running Conditions Reduced Denaturing (4-12 % gradients)
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Apr 27 2012

    Thank you for your enquiry. A general protocol for intracellular staining with this antibody can be found at the following link: http://www.abcam.com/ps/pdf/protocols/flow_intracellular_staining.pdf A second protocol describes indirect stainin...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"