Overview

  • Product nameAnti-IFNGR1 antibody
    See all IFNGR1 primary antibodies
  • Description
    Rabbit polyclonal to IFNGR1
  • Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic non-phosphopeptide derived from human IFNGR1 around the phosphorylation site of threonine 457 (G-YP-D-K-P).

  • Positive control
    • Human brain tissue and COS7 cell extracts.

Applications

Our Abpromise guarantee covers the use of ab61179 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/40000.
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 54 kDa).

Target

  • FunctionReceptor for interferon gamma. Two receptors bind one interferon gamma dimer.
  • Involvement in diseaseDefects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance.
  • Sequence similaritiesBelongs to the type II cytokine receptor family.
    Contains 2 fibronectin type-III domains.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • Post-translational
    modifications
    Phosphorylated at Ser/Thr residues.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antiviral Protein Type II antibody
    • Antiviral protein, type 2 antibody
    • AVP type II antibody
    • AVP, type 2 antibody
    • CD 119 antibody
    • CD119 antibody
    • CD119 antigen antibody
    • CDw119 antibody
    • FLJ45734 antibody
    • IFN gamma R alpha antibody
    • IFN gamma R antibody
    • IFN gamma R1 antibody
    • IFN-gamma receptor 1 antibody
    • IFN-gamma-R1 antibody
    • IFNG R1 antibody
    • IFNGR 1 antibody
    • IFNGR antibody
    • IFNGR1 antibody
    • Immune interferon receptor 1 antibody
    • Immune interferon receptor for antibody
    • INGR1_HUMAN antibody
    • Interferon gamma receptor 1 antibody
    • Interferon gamma receptor alpha chain antibody
    • Interferon gamma receptor alpha chain precursor antibody
    see all

Anti-IFNGR1 antibody images

  • ab61179 at 1/50 - 1/100 dilution staining IFNGR1 in human brain by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
  • All lanes : Anti-IFNGR1 antibody (ab61179) at 1/500 dilution

    Lane 1 : COS7 cell extracts
    Lane 2 : COS7 cell extracts with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 54 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
  • ab61179 staining IFNGR1 in human colon tissue by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Cells were formaldehyde fixed prior to blocking in 10% serum for 2 hours at 21°C. The primary antibody was diluted 1/50 and incubated with the sample for 2 hours at 21°C. Alexa fluor® 594 goat polyclonal, diluted 1/5000, was used as the secondary.

    See Abreview

References for Anti-IFNGR1 antibody (ab61179)

This product has been referenced in:
  • Assani K  et al. IFN-? stimulates autophagy-mediated clearance of Burkholderia cenocepacia in human cystic fibrosis macrophages. PLoS One 9:e96681 (2014). WB . Read more (PubMed: 24798083) »
  • Yu KR  et al. A p38 MAPK-mediated alteration of COX-2/PGE2 regulates immunomodulatory properties in human mesenchymal stem cell aging. PLoS One 9:e102426 (2014). WB ; Mouse . Read more (PubMed: 25090227) »

See all 2 Publications for this product

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