Overview

  • Product name
    Anti-IFT140 antibody
  • Description
    Rabbit polyclonal to IFT140
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat
  • Immunogen

    Synthetic peptide corresponding to N terminal amino acids 108-157 (VLRWSPSGNC LLSGDRLGVL LLWRLDQRGR VQGTPLLKHE YGKHLTHCIF) of human IFT140 (NP_055529).

  • Positive control
    • Fetal brain lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab83183 is purified by peptide affinity chromatography method.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab83183 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 165 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:62500.

Target

  • Relevance
    IFT140 contains 9 TPR repeats and 5 WD repeats. The function of the IFT140 protein remains unknown.
  • Database links
  • Alternative names
    • Gs114 antibody
    • Intraflagellar transport 140 homolog (Chlamydomonas) antibody
    • WD and tetratricopeptide repeats protein 2 antibody
    • WDTC2 antibody

Images

  • Anti-IFT140 antibody (ab83183) at 1 µg/ml (in 5% skim milk / PBS buffer) + fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 165 kDa
    Observed band size : 165 kDa
    Additional bands at : 150 kDa. We are unsure as to the identity of these extra bands.

References

ab83183 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab83183.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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