• Product name
  • Description
    Rabbit polyclonal to IFT20
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 50-80 of Human IFT20, conjugated to KLH.

  • Positive control
    • Mouse lung tissue lysates; Human hepatocarcinoma tissue This antibody gave a positive result when used in the following methanol fixed cell lines: WI 38


  • Form
  • Storage instructions
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab103693 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab103693 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 15 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.


  • Relevance
    Eukaryotic cilia are assembled via intraflagellar transport (IFT) in which large protein particles are motored along ciliary microtubules. The IFT20 subunit of a particle is localized to the Golgi complex, plus the basal body and cilia. In living cells, fluorescently tagged IFT20 is highly dynamic and moves between the Golgi complex and the cilium as well as along ciliary microtubules. It has been suggested that IFT20 functions in the delivery of ciliary membrane proteins from the Golgi complex to the cilium. There are three different isoforms.
  • Cellular localization
    Golgi apparatus. Cilium. Centrosome, centriole.
  • Database links
  • Alternative names
    • hIFT20 antibody
    • Intraflagellar transport 20 homolog (Chlamydomonas) antibody
    • Intraflagellar transport protein 20 like antibody
    • Intraflagellar transport protein IFT20 antibody

Anti-IFT20 antibody images

  • ab103693 stained WI 38 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab103693 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-IFT20 antibody (ab103693) at 1/100 dilution + Mouse lung tissue lysates at 35 µg

    Predicted band size : 15 kDa
  • ab103693, at a 1/50 dilution, staining IFT20 in formalin fixed, paraffin embedded Human hepatocarcinoma tissue by Immunohistochemistry, followed by peroxidase conjugated secondary antibody and DAB staining.

References for Anti-IFT20 antibody (ab103693)

ab103693 has not yet been referenced specifically in any publications.

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