Overview

  • Product name
  • Description
    Mouse polyclonal to IFT20
  • Specificity
    Reactive against mammalian transfected lysate.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    IFT20 (NP_777547, 1 a.a. ~ 148 a.a) full-length human protein.

  • Positive control
    • Transfected 293T golgi apparatus lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab73110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/500 - 1/1000. Detects a band of approximately 16 kDa.

    Detection antibody.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Eukaryotic cilia are assembled via intraflagellar transport (IFT) in which large protein particles are motored along ciliary microtubules. The IFT20 subunit of a particle is localized to the Golgi complex, plus the basal body and cilia. In living cells, fluorescently tagged IFT20 is highly dynamic and moves between the Golgi complex and the cilium as well as along ciliary microtubules. It has been suggested that IFT20 functions in the delivery of ciliary membrane proteins from the Golgi complex to the cilium. There are three different isoforms.
    • Cellular localization
      Golgi apparatus. Cilium. Centrosome, centriole.
    • Database links
    • Alternative names
      • hIFT20 antibody
      • Intraflagellar transport 20 homolog (Chlamydomonas) antibody
      • Intraflagellar transport protein 20 like antibody
      • Intraflagellar transport protein IFT20 antibody

    Anti-IFT20 antibody images

    • All lanes : Anti-IFT20 antibody (ab73110) at 1/500 dilution

      Lane 1 : IFT20 transfected 293T cell line
      Lane 2 : Non transfected 293T cell line

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Observed band size : 16 kDa (why is the actual band size different from the predicted?)

    References for Anti-IFT20 antibody (ab73110)

    This product has been referenced in:
    • Mandell MA  et al. TRIM17 contributes to autophagy of midbodies while actively sparing other targets from degradation. J Cell Sci 129:3562-3573 (2016). Read more (PubMed: 27562068) »

    See 1 Publication for this product

    Product Wall

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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