Anti-IFT43 antibody (ab121195)
Key features and details
- Rabbit polyclonal to IFT43
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IFT43 antibody -
Description
Rabbit polyclonal to IFT43 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to amino acids 107-202 (EEVQEEDFVL QVAAPPSIQI KRVMTYRDLD NDLMKYSAIQ TLDGEIDLKL LTKVLAPEHE VREDDVGWDW DHLFTEVSSE VLTEWDPLQT EKEDPA) of Human IFT43 (Q96FT9).
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Positive control
- IFT43-transfected HEK293T lysate; Human testis tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121195 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
Target
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Function
Component of IFT complex A (IFT-A) involved in retrograde ciliary transport along microtubules from the ciliary tip to the base. -
Involvement in disease
Defects in IFT43 are a cause of cranioectodermal dysplasia type 1 (CED1) [MIM:218330]. CED1 is a disorder characterized by craniofacial, skeletal and ectodermal abnormalities. Clinical features include dolichocephaly (with or without sagittal suture synostosis), scaphocephaly, short stature, limb shortening, short ribs, narrow chest, brachydactyly, renal failure and hepatic fibrosis, small and abnormally shaped teeth, sparse hair, skin laxity and abnormal nails. -
Sequence similarities
Belongs to the IFT43 family. - Information by UniProt
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Database links
- Entrez Gene: 112752 Human
- Omim: 614068 Human
- SwissProt: Q96FT9 Human
- Unigene: 532626 Human
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Alternative names
- C14orf179 antibody
- ift43 antibody
- IFT43_HUMAN antibody
- Intraflagellar transport protein 43 homolog antibody
Images
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Immunofluorescent staining of Human cell line U-251MG shows positivity in cytoskeleton. Recommended concentration of ab121195 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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All lanes : Anti-IFT43 antibody (ab121195) at 1/250 dilution
Lane 1 : Vector only transfected HEK293T lysate
Lane 2 : IFT43-transfected HEK293T lysate (Co-expressed with a C-terminal myc-DDK tag ~3.1 kDa)
Developed using the ECL technique. -
ab121195 at 1/50 dilution staining IFT43 in Paraffin-embedded Human testis tissue by Immunohistochemistry.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab121195 has not yet been referenced specifically in any publications.