Overview

  • Product nameAnti-IFT43 antibody
  • Description
    Rabbit polyclonal to IFT43
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 107-202 (EEVQEEDFVL QVAAPPSIQI KRVMTYRDLD NDLMKYSAIQ TLDGEIDLKL LTKVLAPEHE VREDDVGWDW DHLFTEVSSE VLTEWDPLQT EKEDPA) of Human IFT43 (Q96FT9).

  • Positive control
    • IFT43-transfected HEK293T lysate; Human testis tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab121195 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

  • FunctionComponent of IFT complex A (IFT-A) involved in retrograde ciliary transport along microtubules from the ciliary tip to the base.
  • Involvement in diseaseDefects in IFT43 are a cause of cranioectodermal dysplasia type 1 (CED1) [MIM:218330]. CED1 is a disorder characterized by craniofacial, skeletal and ectodermal abnormalities. Clinical features include dolichocephaly (with or without sagittal suture synostosis), scaphocephaly, short stature, limb shortening, short ribs, narrow chest, brachydactyly, renal failure and hepatic fibrosis, small and abnormally shaped teeth, sparse hair, skin laxity and abnormal nails.
  • Sequence similaritiesBelongs to the IFT43 family.
  • Information by UniProt
  • Database links
  • Alternative names
    • C14orf179 antibody
    • ift43 antibody
    • IFT43_HUMAN antibody
    • Intraflagellar transport protein 43 homolog antibody

Anti-IFT43 antibody images

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in cytoskeleton. Recommended concentration of ab121195 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-IFT43 antibody (ab121195) at 1/250 dilution

    Lane 1 : Vector only transfected HEK293T lysate
    Lane 2 : IFT43-transfected HEK293T lysate (Co-expressed with a C-terminal myc-DDK tag ~3.1 kDa)

    Developed using the ECL technique
  • ab121195 at 1/50 dilution staining IFT43 in Paraffin-embedded Human testis tissue by Immunohistochemistry.

References for Anti-IFT43 antibody (ab121195)

ab121195 has not yet been referenced specifically in any publications.

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