Overview

  • Product name
  • Description
    Rabbit polyclonal to IFT46
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: ETDSDSDDDD EEHGAPLEGA YDPADYEHLP VSAEIKELFQ YISRYTPQLI DLDHKLKPFI PDFIPAVGDI DAFLKVPRPD GKPDNLGLLV, corresponding to amino acids 44-133 of Human IFT46.

  • Positive control
    • Human bronchus tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab122422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Forms part of a complex involved in intraflagellar transport (IFT), the bi-directional movement of particles required for the assembly, maintenance and functioning of primary cilia. May play a role in chondrocyte maturation and skeletogenesis.
  • Sequence similarities
    Belongs to the IFT46 family.
  • Cellular localization
    Cytoplasm > cytoskeleton > cilium basal body. Cell projection > cilium. Expression is concentrated at the cilium basal body but is also detected along the length of the cilium.
  • Information by UniProt
  • Database links
  • Alternative names
    • C11orf2 antibody
    • C11orf60 antibody
    • IFT46 antibody
    • IFT46_HUMAN antibody
    • Intraflagellar transport protein 46 homolog antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-251 MG shows positivity in cytoplasm. Recommended concentration of ab122422 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122422 at 1/1000 dilution staining IFT46 in Paraffin-embedded Human bronchus tissue by Immunohistochemistry.

References

ab122422 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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