The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionForms part of a complex involved in intraflagellar transport (IFT), the bi-directional movement of particles required for the assembly, maintenance and functioning of primary cilia. May play a role in chondrocyte maturation and skeletogenesis.
Sequence similaritiesBelongs to the IFT46 family.
Cellular localizationCytoplasm > cytoskeleton > cilium basal body. Cell projection > cilium. Expression is concentrated at the cilium basal body but is also detected along the length of the cilium.