The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Component of the intraflagellar transport (IFT) complex B, which is essential for the development and maintenance of motile and sensory cilia.
Involvement in disease
Defects in IFT80 are the cause of asphyxiating thoracic dystrophy type 2 (ATD2) [MIM:611263]. An autosomal recessive chondrodysplasia characterized by a severely constricted thoracic cage, short-limbed short stature, and polydactyly. It often leads to death in infancy because of respiratory insufficiency. Retinal degeneration, cystic renal disease and hepatic disease can be present in affected individuals who survive early childhood.
Contains 7 WD repeats.
Cytoplasm. Cytoplasm > cytoskeleton > cilium basal body. Cytoplasm > cytoskeleton > cilium axoneme. Basal body and ciliary axoneme.