Overview

  • Product name
  • Description
    Rabbit polyclonal to IFT80
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: EELYSCSDDH QIVKWNLLTS ETTQIVKLPD DIYPIDFHWF PKSLGVKKQT QAESFVLTSS DGKFHLISKL GRVEKSVEAH CGAVLAGRWN Y, corresponding to amino acids 26-116 of Human IFT80.

  • Positive control
    • Human kidney tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab126784 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Component of the intraflagellar transport (IFT) complex B, which is essential for the development and maintenance of motile and sensory cilia.
  • Involvement in disease
    Defects in IFT80 are the cause of asphyxiating thoracic dystrophy type 2 (ATD2) [MIM:611263]. An autosomal recessive chondrodysplasia characterized by a severely constricted thoracic cage, short-limbed short stature, and polydactyly. It often leads to death in infancy because of respiratory insufficiency. Retinal degeneration, cystic renal disease and hepatic disease can be present in affected individuals who survive early childhood.
  • Sequence similarities
    Contains 7 WD repeats.
  • Cellular localization
    Cytoplasm. Cytoplasm > cytoskeleton > cilium basal body. Cytoplasm > cytoskeleton > cilium axoneme. Basal body and ciliary axoneme.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATD2 antibody
    • Ift80 antibody
    • IFT80_HUMAN antibody
    • Intraflagellar transport 80 homolog (Chlamydomonas) antibody
    • Intraflagellar transport protein 80 homolog antibody
    • KIAA1374 antibody
    • WD repeat domain 56 antibody
    • WD repeat-containing protein 56 antibody
    • WDR56 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus and nucleoli. Recommended concentration of ab126784 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab126784, at 1/50 dilution, staining IFT80 in formalin-fixed, paraffin-embedded Human kidney tissue, by Immunohistochemistry.

References

ab126784 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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