Recombinant
RabMAb

Anti-IgA antibody [EPR5367-76] (ab124716)

Overview

  • Product name
    Anti-IgA antibody [EPR5367-76]
    See all IgA primary antibodies
  • Description
    Rabbit monoclonal [EPR5367-76] to IgA
  • Specificity
    ab124716 reacts with Heavy chain of IgA. It does not react with either kappa or lambda light chain. It does not react with other Ig.
  • Tested applications
    Suitable for: WB, IP, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified Human IgA

  • Positive control
    • Human tonsil, Human plasma, and Human spleen lysates; Human colon tissue, Human tonsil tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR5367-76
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab124716 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
IP 1/10 - 1/100.
ELISA 1/250 - 1/4000.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.

Target

  • Relevance
    Human IgA (immunoglobulin A) is a glycosylated protein of 160 kDa and is produced as a monomer or as a J chain linked dimer. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces such as saliva, gastrointestinal secretion, bronchial fluids and milk. Mucosal IgA plays a major role in host defence by neutralising infectious agents at mucosal surfaces. The production is usually local and antigen specific IgA producing B cells can be found in regions under the lamina propria where they mature into dimeric IgA producing plasma cells. IgA deficiency is the most common immunodeficiency that may affect both serum and mucosal produced IgA. OR: The secretory component is a component of immunoglobulin A (IgA) which consists of a portion of the polymeric immunoglobulin receptor. Polymeric IgA binds to the polymeric immunoglobulin receptor on the basolateral surface of epithelial cells and is taken up into the cell via transcytosis. The receptor-IgA complex passes through the cellular compartments before being secreted on the luminal surface of the epithelial cells, still attached to the receptor. Proteolysis of the receptor occurs and the dimeric IgA molecule, along with the secretory component, are free to diffuse throughout the lumen.
  • Cellular localization
    Secreted
  • Database links
  • Alternative names
    • Hepatocellular carcinoma-associated protein TB6 antibody
    • Ig alpha 1 chain C region antibody
    • Ig alpha 2 chain C region antibody
    • IGHA antibody
    • IGHA1 antibody
    • IGHA2 antibody
    • Immunoglobulin heavy constant alpha 1 antibody
    • Immunoglobulin heavy constant alpha 2 A2m marker antibody
    • Immunoglobulin heavy constant alpha 2 antibody
    • PIgR antibody
    • Poly-Ig receptor antibody
    • polymeric immunoglobulin receptor antibody
    • polymeric immunoglobulin receptor Secretory component antibody
    see all

Images

  • Capture ab 1:60 dilution (1μg in 1mg lysate). Primary ab for WB ab124716 at 1:1000 dilution (0.18μg/ml). Secondary ab Anti-Rabbit IgG (HRP), specific to the non5% NFDM/TBST -reduced form of IgG. Secondary ab concentration 1:1500 dilution. Blocking buffer and concentration 5% NFDM/TBST. Diluting buffer and concentration 5% NFDM/TBST Lane 1 (+) Human plasma Lane 2 (-) Rabbit monoclonal IgG (ab172730) instead of ab124716 in Human plasma

  • ab124716, at 1/100, staining IgA in paraffin embedded Human colon tissue by Immunohistochemistry.
  • ab124716, at 1/100, staining IgA in paraffin embedded Human tonsil tissue by Immunohistochemistry.
  • All lanes : Anti-IgA antibody [EPR5367-76] (ab124716) at 1/1000 dilution

    Lane 1 : Human tonsil lysate
    Lane 2 : Human plasma lysate
    Lane 3 : Human spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 37 kDa

References

This product has been referenced in:
  • Fahrbach KM  et al. Differential binding of IgG and IgA to mucus of the female reproductive tract. PLoS One 8:e76176 (2013). WB ; Human . Read more (PubMed: 24098437) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (PBMC)
Permeabilization
Yes - 4x Cytofix, 45 min, 4 ¯C
Gating Strategy
CD19 positive live cells
Specification
PBMC
Preparation
Cell harvesting/tissue preparation method: PBMC from whole blood by density gradient (lymphoprep, Fresenius)
Sample buffer: D-PBS+5 % FCS + 2 mM EDTA
Username

Clara Lettl

Verified customer

Submitted Jan 18 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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