Overview

  • Product nameAnti-IgA antibody [EPR5367-76]
    See all IgA primary antibodies
  • Description
    Rabbit monoclonal [EPR5367-76] to IgA
  • Tested applicationsSuitable for: WB, IP, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified Human IgA

  • Positive control
    • Human tonsil, Human plasma, and Human spleen lysates; Human colon tissue, Human tonsil tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberEPR5367-76
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124716 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
IP 1/10 - 1/100.
ELISA 1/250 - 1/4000.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.

Target

  • RelevanceHuman IgA (immunoglobulin A) is a glycosylated protein of 160 kDa and is produced as a monomer or as a J chain linked dimer. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces such as saliva, gastrointestinal secretion, bronchial fluids and milk. Mucosal IgA plays a major role in host defence by neutralising infectious agents at mucosal surfaces. The production is usually local and antigen specific IgA producing B cells can be found in regions under the lamina propria where they mature into dimeric IgA producing plasma cells. IgA deficiency is the most common immunodeficiency that may affect both serum and mucosal produced IgA. OR: The secretory component is a component of immunoglobulin A (IgA) which consists of a portion of the polymeric immunoglobulin receptor. Polymeric IgA binds to the polymeric immunoglobulin receptor on the basolateral surface of epithelial cells and is taken up into the cell via transcytosis. The receptor-IgA complex passes through the cellular compartments before being secreted on the luminal surface of the epithelial cells, still attached to the receptor. Proteolysis of the receptor occurs and the dimeric IgA molecule, along with the secretory component, are free to diffuse throughout the lumen.
  • Cellular localizationSecreted
  • Database links
  • Alternative names
    • Hepatocellular carcinoma-associated protein TB6 antibody
    • Ig alpha 1 chain C region antibody
    • Ig alpha 2 chain C region antibody
    • IGHA antibody
    • IGHA1 antibody
    • IGHA2 antibody
    • Immunoglobulin heavy constant alpha 1 antibody
    • Immunoglobulin heavy constant alpha 2 A2m marker antibody
    • Immunoglobulin heavy constant alpha 2 antibody
    • PIgR antibody
    • Poly-Ig receptor antibody
    • polymeric immunoglobulin receptor antibody
    • polymeric immunoglobulin receptor Secretory component antibody
    see all

Anti-IgA antibody [EPR5367-76] images

  • ab124716, at 1/100, staining IgA in paraffin embedded Human colon tissue by Immunohistochemistry.
  • ab124716, at 1/100, staining IgA in paraffin embedded Human tonsil tissue by Immunohistochemistry.
  • All lanes : Anti-IgA antibody [EPR5367-76] (ab124716) at 1/1000 dilution

    Lane 1 : Human tonsil lysate
    Lane 2 : Human plasma lysate
    Lane 3 : Human spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 37 kDa

References for Anti-IgA antibody [EPR5367-76] (ab124716)

This product has been referenced in:
  • Fahrbach KM  et al. Differential binding of IgG and IgA to mucus of the female reproductive tract. PLoS One 8:e76176 (2013). WB ; Human . Read more (PubMed: 24098437) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"