Anti-IgA antibody [IgA5-3B] (Biotin) (ab53270)

Overview

  • Product name
    Anti-IgA antibody [IgA5-3B] (Biotin)
    See all IgA primary antibodies
  • Description
    Mouse monoclonal [IgA5-3B] to IgA (Biotin)
  • Conjugation
    Biotin
  • Specificity
    ab53270 recognises IgA. It does not cross-react with IgG or IgM; it has been shown to detect secreted IgA in cat saliva and rhesus monkey jejunal washes
  • Tested applications
    Suitable for: ELISAmore details
  • Species reactivity
    Reacts with: Cat, Dog, Human, Rhesus monkey, Common marmoset
  • Immunogen

    Purified monkey IgA, from serum.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    ab53270 is prepared by affinity chromatography on Protein A from tissue culture supernatant.
  • Clonality
    Monoclonal
  • Clone number
    IgA5-3B
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
  • Application notes
    ELISA: Use at an assay dependent dilution. 1 - 10 ug/ml is a good starting point for ELISA.



    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Human IgA (immunoglobulin A) is a glycosylated protein of 160 kDa and is produced as a monomer or as a J chain linked dimer. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces such as saliva, gastrointestinal secretion, bronchial fluids and milk. Mucosal IgA plays a major role in host defence by neutralising infectious agents at mucosal surfaces. The production is usually local and antigen specific IgA producing B cells can be found in regions under the lamina propria where they mature into dimeric IgA producing plasma cells. IgA deficiency is the most common immunodeficiency that may affect both serum and mucosal produced IgA. OR: The secretory component is a component of immunoglobulin A (IgA) which consists of a portion of the polymeric immunoglobulin receptor. Polymeric IgA binds to the polymeric immunoglobulin receptor on the basolateral surface of epithelial cells and is taken up into the cell via transcytosis. The receptor-IgA complex passes through the cellular compartments before being secreted on the luminal surface of the epithelial cells, still attached to the receptor. Proteolysis of the receptor occurs and the dimeric IgA molecule, along with the secretory component, are free to diffuse throughout the lumen.
    • Cellular localization
      Secreted
    • Database links
    • Alternative names
      • Hepatocellular carcinoma-associated protein TB6 antibody
      • Ig alpha 1 chain C region antibody
      • Ig alpha 2 chain C region antibody
      • IGHA antibody
      • IGHA1 antibody
      • IGHA2 antibody
      • Immunoglobulin heavy constant alpha 1 antibody
      • Immunoglobulin heavy constant alpha 2 A2m marker antibody
      • Immunoglobulin heavy constant alpha 2 antibody
      • PIgR antibody
      • Poly-Ig receptor antibody
      • polymeric immunoglobulin receptor antibody
      • polymeric immunoglobulin receptor Secretory component antibody
      see all

    References

    ab53270 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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