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Full length native protein (purified) corresponding to Human IgA Secretory Component. Affinity-purified secretory component from human colostrum.
Examples of positive human tissues: secretory mucosa of gastrointestinal and respiratory tract, epithelia of salivary glands, endometrium, endocervix, kidney, prostate, mammary gland.
Our Abpromise guarantee covers the use of ab3924 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 61 kDa). See Abreview. Use under non-reducing conditions.|
|IHC-P||Use at an assay dependent concentration.|
ab3924 staining IgA Secretory Component in human normal breast tissue by Immunohistochemistry (Frozen sections).Tissue was fixed in methanol, blocked using 10% serum for 5 minutes at 25°C and then incubated with ab3924 at a 1/100 dilution for 1 hour at 25°C. The secondary used was an undiluted HRP conjugated goat polyclonal.The images show stainings of normal breast glands from two different patients. The antibody mostly stains the apical/luminal region of luminal epithelial cells, but the intensity varies from patient to patient.
Immunohistochemical analysis of Human breast cancer tissue, staining IgA Secretory Component with ab3924.
Antigen retrieval was performed by heat mediation in a citrate buffer (pH 6). Tissue was blocked with 10% normal goat serum before incubating with primary antibody (1/50) for 2 hours at room temperature. Staining was primarily in the cytoplasm.