Anti-IgA Secretory Component antibody [SC-05 COMPO2] (ab17377)

Overview

  • Product nameAnti-IgA Secretory Component antibody [SC-05 COMPO2]
    See all IgA Secretory Component primary antibodies
  • Description
    Mouse monoclonal [SC-05 COMPO2] to IgA Secretory Component
  • SpecificityAb17377 recognizes a human glycoprotein, identified as secretory component. Reacts with both free and polymeric Ig-bound secretory component. Shows no reaction in Western Blots of cell lines lacking secretory component. The clone number has been updated from (0.N.556) to (SC-05 COMPO2) both clone numbers name the same antibody clone.
  • Tested applicationsSuitable for: IHC-P, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Full length protein corresponding to Human IgA Secretory Component. Semi-purified human secretory component

  • Positive control
    • Small intestine, stomach, colon carcinoma

Properties

Applications

Our Abpromise guarantee covers the use of ab17377 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use a concentration of 1 µg/ml.
ICC/IF Use at an assay dependent concentration.

Target

  • RelevanceIgA has two subclasses (IgA1 and IgA2) and can be produced as a monomeric as well as a dimeric form. The IgA dimeric form is the most prevalent and is also called secretory IgA (sIgA). The secretory component of sIgA protects the immunoglobulin from being degraded by proteolytic enzymes, thus sIgA can survive in the harsh gastrointestinal tract environment and provide protection against microbes that multiply in body secretions. sIgA can also inhibit inflammatory effects of other immunoglobulins. Secretory component is a proteolytic cleavage product of the polymeric immunoglobulin receptor which remains associated with dimeric IgA in sero-mucus secretions. Polymeric IgA binds to the polymeric immunoglobulin receptor on the basolateral surface of epithelial cells and is taken up into the cell via transcytosis. The receptor-IgA complex passes through the cellular compartments before being secreted on the luminal surface of the epithelial cells, still attached to the receptor. Proteolysis of the receptor occurs and the dimeric IgA molecule, along with the secretory component, are free to diffuse throughout the lumen. Secretory components wrap around two IgA units joined by a J chain protein fragment, resulting in a >--< configuration, with each of the two antigen binding regions of the two constituent y-shaped antibodies exposed. One identified function of secretory components is to protect IgA antibodies from degradation by the gastric acids and enzymes of the digestive system. This property is especially important in the transfer of immune system components during breastfeeding.

References for Anti-IgA Secretory Component antibody [SC-05 COMPO2] (ab17377)

ab17377 has not yet been referenced specifically in any publications.

Product Wall

I have had a look at the nature of this protein: Extracellular matrix protein 1 (http://www.uniprot.org/uniprot/Q16610)

Unfortunately, I do not think it is scientifically to detect only ECM1b without detecting ECM1a.

The difference ...

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Application Western blot
Loading amount 1 µg
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Sample Human Recombinant protein (293T cells)
Specification 293T cells
Username

Dr. Kye Hanafiah

Verified customer

Submitted Apr 18 2014

DISCOUNT CODES:

For Ab17377: ***
For Ab96196: ***

Expiration date: February 18, 2013

Value: $337 each

Each of these codes will give you $337 off your next order before the expiration date. To redeem this off...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"