Overview

  • Product nameAnti-IgA2 antibody
    See all IgA2 primary antibodies
  • Description
    Mouse polyclonal to IgA2
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human IgA2 protein (AAH73765.1).

  • Positive control
    • WB: Human colon tissue lysate; Hela nuclear extract; IgA2 transfected 293T cell lysate ICc/IF: HeLa cell

Properties

Applications

Our Abpromise guarantee covers the use of ab88250 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 53 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • RelevanceIg alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system.
  • Cellular localizationSecreted
  • Database links
  • Alternative names
    • A2m marker antibody
    • Ig alpha 2 chain C region antibody
    • IgA constant heavy chain 2 antibody
    • IGHA 2 antibody
    • IGHA2 antibody
    • IGHA2 protein antibody
    • Immunoglobulin A2 antibody
    • Immunoglobulin Am2 antibody
    • immunoglobulin heavy constant alpha 2 (A2m marker) antibody
    • Immunoglobulin heavy constant alpha 2 antibody
    see all

Anti-IgA2 antibody images

  • Anti-IgA2 antibody (ab88250) at 1 µg/ml + human colon tissue lysate at 50 µg

    Predicted band size : 53 kDa
    Observed band size : 53 kDa
  • Anti-IgA2 antibody (ab88250) at 1 µg/ml + Hela nuclear extract at 50 µg

    Predicted band size : 53 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-IgA2 antibody (ab88250) at 1 µg/ml

    Lane 1 : IgA2 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 50 µg per lane.


    Predicted band size : 53 kDa
    Observed band size : 53 kDa
  • ab88250, at 10 µg/ml, staining IgA2 in HeLa cell by Immunofluorescence.

References for Anti-IgA2 antibody (ab88250)

ab88250 has not yet been referenced specifically in any publications.

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