Overview

  • Product nameAnti-IGBP1 antibody
    See all IGBP1 primary antibodies
  • Description
    Rabbit polyclonal to IGBP1
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide from a region between residue 300 and the C-terminus (residue 339) of human IGBP1 using the numbering given in SwissProt entry P78318

  • Positive control
    • Whole cell lysate from Ramos, K562 and NIH3T3 cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7 to 8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab70545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionAssociated to surface IgM-receptor; may be involved in signal transduction. May be involved in regulation of the catalytic activity of type 2A-related serine/threonine phosphatases.
  • Tissue specificityUbiquitously expressed with highest levels in heart, skeletal muscle and pancreas.
  • Involvement in diseaseDefects in IGBP1 are the cause of agenesis of the corpus callosum with mental retardation-ocular coloboma-micrognathia (ACCMRCM) [MIM:300472].
  • Sequence similaritiesBelongs to the IGBP1/TAP42 family.
  • Post-translational
    modifications
    Phosphorylated.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha 4 antibody
    • B cell signal transduction molecule alpha 4 antibody
    • B-cell signal transduction molecule alpha 4 antibody
    • bA351K23.1 (immunoglobulin binding protein 1 (CD79A) ) antibody
    • bA351K23.1 antibody
    • CD79a binding protein 1 antibody
    • CD79a-binding protein 1 antibody
    • IBP 1 antibody
    • IBP1 antibody
    • IGBP 1 antibody
    • Igbp1 antibody
    • IGBP1_HUMAN antibody
    • Immunoglobulin (CD79A) binding protein 1 antibody
    • Immunoglobulin binding protein 1 antibody
    • Immunoglobulin-binding protein 1 antibody
    • Protein alpha 4 antibody
    • Protein alpha-4 antibody
    • Protein phosphatase 2/4/6 regulatory subunit antibody
    • Protein phosphatase 2A regulatory subunit alpha 4 antibody
    • Renal carcinoma antigen NY REN 16 antibody
    • Renal carcinoma antigen NY-REN-16 antibody
    see all

Anti-IGBP1 antibody images

  • All lanes : Anti-IGBP1 antibody (ab70545) at 0.4 µg/ml

    Lane 1 : Whole cell lysate from Ramos cells
    Lane 2 : Whole cell lysate from K562 cells
    Lane 3 : Whole cell lysate from NIH3T3 cells

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique

    Predicted band size : 39 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds
  • 1mg whole cell lysate from Ramos cells was immunoprecipitated with ab70545 at 3ug/mg of lysate (lane 1) or a control rabbit Ig (lane 2). For the subsequent blot 25% of the immunoprecipitate was loaded per lane, and probed with ab70545 at 1ug/ml.

References for Anti-IGBP1 antibody (ab70545)

ab70545 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Adipocyte)
Loading amount 5 µg
Specification Adipocyte
Gel Running Conditions Reduced Denaturing (12% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Dec 15 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"