Overview

  • Product name
    Anti-IgE antibody [4C3]
    See all IgE primary antibodies
  • Description
    Mouse monoclonal [4C3] to IgE
  • Specificity
    ab106494 is specific for Human IgE. It reacts with IgE purified from blood but does not cross-react with Human anti-NP IgG1, IgG2, IgG3, IgG4, IgA and IgM
  • Tested applications
    Suitable for: WB, ELISA, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse
  • Immunogen

    Mouse-Human chimeric anti-NP IgE

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    4C3
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab106494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa.
ELISA Use a concentration of 1 µg/ml. Can be paired for ELISA with Mouse monoclonal [1A2] to IgE (HRP) (ab106493). as primary antibody
Sandwich ELISA Use a concentration of 5 µg/ml. for coating plates. Use ab106493 for detection.

Target

  • Relevance
    IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock.
  • Cellular localization
    Cell Membrane
  • Database links
  • Alternative names
    • Igh2 antibody
    • IGHE antibody
    • IGHEP1 antibody
    • Immunoglobulin heavy constant epsilon antibody

Images

  • ab106494 used at 5 µg/ml for coating ELISA plates to capture Human IgE. Detection utilised ab106493 at 1/1000 dilution.

References

ab106494 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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