Anti-IgE antibody [4F4] (HRP) (ab7294)

Overview

  • Product name
    Anti-IgE antibody [4F4] (HRP)
    See all IgE primary antibodies
  • Description
    Mouse monoclonal [4F4] to IgE (HRP)
  • Conjugation
    HRP
  • Specificity
    IgE Ce3-domain specific.
  • Tested applications
    Suitable for: ELISA, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) corresponding to Human IgE. IgE from myelomatous human serum.

  • General notes
    Concentration varies from lot to lot and can be provided on request.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.05% Proclin
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    Purified by chromatography on protein A Sepharose. Purity is tested by electrophoresis.
  • Clonality
    Monoclonal
  • Clone number
    4F4
  • Myeloma
    Sp2/0
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7294 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution. Ab7294 can be used for the detection of total IgE level and IgE antibodies in ELISA
AP Use at an assay dependent dilution.
Sandwich ELISA Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [5D4] to IgE (ab7295). Can be used as Detection antibody with recommended pair.

Target

  • Relevance
    IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock.
  • Cellular localization
    Cell Membrane
  • Database links
  • Alternative names
    • Igh2 antibody
    • IGHE antibody
    • IGHEP1 antibody
    • Immunoglobulin heavy constant epsilon antibody

References

ab7294 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting us and sorry for the delay in getting back to you.

In answer to your questions:

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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