Overview

  • Product nameAnti-IgE antibody [4G7]
    See all IgE primary antibodies
  • Description
    Mouse monoclonal [4G7] to IgE
  • SpecificityThis antibody reacts with human IgE. Specificity was confirmed by ELISA
  • Tested applicationsELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) corresponding to Human IgE.

  • General notesThis antibody recognizes an epitope different from the one recognized by ab774 and ab775.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity>95% by SDS-PAGE
  • Purification notesPurified from ascites by DEAE chromatography and precipitation methods.
  • Clonality Monoclonal
  • Clone number4G7
  • Myelomaunknown
  • IsotypeIgG
  • Light chain typeunknown
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
  • Application notesELISA: Use at an assay dependant dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceIgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock.
    • Cellular localizationCell Membrane
    • Database links
    • Alternative names
      • Ig epsilon chain C region antibody
      • IGHE antibody
      • Immunoglobulin epsilon antibody
      • Immunoglobulin heavy constant epsilon antibody

    References for Anti-IgE antibody [4G7] (ab773)

    This product has been referenced in:
    • Mueller GA  et al. Hydrogen exchange nuclear magnetic resonance spectroscopy mapping of antibody epitopes on the house dust mite allergen Der p 2. J Biol Chem 276:9359-65 (2001). Read more (PubMed: 11134039) »
    • Smith AM  et al. The molecular basis of antigenic cross-reactivity between the group 2 mite allergens. J Allergy Clin Immunol 107:977-84 (2001). Read more (PubMed: 11398074) »

    See all 2 Publications for this product

    Product Wall

    Detailed epitope mapping was not performed (only cross-blocking studies with the three mAb to human IgE). Conjugates with HRP will be available, most probably will appear in the catalogue on September/October 2002. The preliminary studies showed that H...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"