Overview

  • Product nameAnti-IgE antibody [4H10]
    See all IgE primary antibodies
  • Description
    Mouse monoclonal [4H10] to IgE
  • SpecificityThis antibody reacts with human IgE. This antibody recognizes an epitope different from the one recognized by ab773 and ab775. Specificity was confirmed by ELISA
  • Tested applicationsSuitable for: Flow Cyt, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) corresponding to Human IgE.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: Tris buffered saline, pH 8.0
  • Concentration information loading...
  • Purity>95% by SDS-PAGE
  • Purification notesPurified from ascites by DEAE chromatography and precipitation methods.
  • ClonalityMonoclonal
  • Clone number4H10
  • Myelomaunknown
  • IsotypeIgG1
  • Light chain typeunknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab774 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 4 µg/ml.
ELISA Use at an assay dependent concentration.

Target

  • RelevanceIgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock.
  • Cellular localizationCell Membrane
  • Database links
  • Alternative names
    • Igh2 antibody
    • IGHE antibody
    • IGHEP1 antibody
    • Immunoglobulin heavy constant epsilon antibody

References for Anti-IgE antibody [4H10] (ab774)

ab774 has not yet been referenced specifically in any publications.

Product Wall

This antibody (ab774) has not been shown to be a IgE neutralizing body. I suggest that you conduct a search through the Abcam Website. I also recommend that you should click on the links “Product Reviews” and “Technical Enquiries” both accessible via t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"