Overview

  • Product nameAnti-IgE antibody [4H10] (FITC)
    See all IgE primary antibodies
  • Description
    Mouse monoclonal [4H10] to IgE (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified human IgE.

  • General notes

    The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 0.2% BSA (high-grade protease free), PBS
  • Concentration information loading...
  • PuritySize exclusion
  • ClonalityMonoclonal
  • Clone number4H10
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 2 - 5 µg/ml. ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • RelevanceIgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock.
  • Cellular localizationCell Membrane
  • Database links
  • Alternative names
    • Igh2 antibody
    • IGHE antibody
    • IGHEP1 antibody
    • Immunoglobulin heavy constant epsilon antibody

Anti-IgE antibody [4H10] (FITC) images

  • ab77239 staining IgE in Human basophil granulocytes by Flow Cytometry. Cells were peripheral blood cells, separated by Ficoll density separation, followed by magnetic isolation. The sample was incubated with the primary antibody (3μg/ml in PBS with Human IgG (Fc-block)) for 45 minutes at 4°C.

    See Abreview

References for Anti-IgE antibody [4H10] (FITC) (ab77239)

This product has been referenced in:
  • Gervais FG  et al. Pharmacological characterization of MK-7246, a potent and selective CRTH2 (chemoattractant receptor-homologous molecule expressed on T-helper type 2 cells) antagonist. Mol Pharmacol 79:69-76 (2011). Flow Cyt ; Human . Read more (PubMed: 20943773) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Fixation no fixation
Permeabilization No
Sample Human Cell (human basophil granulocytes)
Specification human basophil granulocytes
Gating Strategy no gate
Preparation Cell harvesting/tissue preparation method: peripheral blood cells, separated by Ficoll density separation, followed by magnetic isolation
Sample buffer: PBS with human IgG (Fc-block)
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Submitted Aug 15 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"