Overview

  • Product name
    Anti-IgE antibody [4H10] (FITC)
    See all IgE primary antibodies
  • Description
    Mouse monoclonal [4H10] to IgE (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified human IgE.

  • General notes

    The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 15mM Sodium Azide
    Constituents: 0.2% BSA (high-grade protease free), PBS
  • Concentration information loading...
  • Purity
    Size exclusion
  • Clonality
    Monoclonal
  • Clone number
    4H10
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 2 - 5 µg/ml. ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Relevance
    IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock.
  • Cellular localization
    Cell Membrane
  • Database links
  • Alternative names
    • Igh2 antibody
    • IGHE antibody
    • IGHEP1 antibody
    • Immunoglobulin heavy constant epsilon antibody

Anti-IgE antibody [4H10] (FITC) images

  • ab77239 staining IgE in Human basophil granulocytes by Flow Cytometry. Cells were peripheral blood cells, separated by Ficoll density separation, followed by magnetic isolation. The sample was incubated with the primary antibody (3μg/ml in PBS with Human IgG (Fc-block)) for 45 minutes at 4°C.

    See Abreview

References for Anti-IgE antibody [4H10] (FITC) (ab77239)

This product has been referenced in:
  • Gervais FG  et al. Pharmacological characterization of MK-7246, a potent and selective CRTH2 (chemoattractant receptor-homologous molecule expressed on T-helper type 2 cells) antagonist. Mol Pharmacol 79:69-76 (2011). Flow Cyt ; Human . Read more (PubMed: 20943773) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Fixation
no fixation
Permeabilization
No
Sample
Human Cell (human basophil granulocytes)
Specification
human basophil granulocytes
Gating Strategy
no gate
Preparation
Cell harvesting/tissue preparation method: peripheral blood cells, separated by Ficoll density separation, followed by magnetic isolation
Sample buffer: PBS with human IgG (Fc-block)
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Verified customer

Submitted Aug 15 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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