• Product nameAnti-IgE antibody [5D4]
    See all IgE primary antibodies
  • Description
    Mouse monoclonal [5D4] to IgE
  • SpecificitySpecific for IgE Ce2-domain specific
  • Tested applicationsSuitable for: ELISA, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) corresponding to Human IgE. IgE from myelomatous human serum.

  • General notesConcentration varies from lot to lot and can be provided on request.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesPurified by chromatography on protein A Sepharose. Purity is tested by electrophoresis.
  • ClonalityMonoclonal
  • Clone number5D4
  • MyelomaSp2/0
  • IsotypeIgG2a
  • Research areas


Our Abpromise guarantee covers the use of ab7295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution. Ab7295 can be used for the detection of total IgE levels and IgE antibodies by ELISA
Sandwich ELISA Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [4F4] to IgE (HRP) (ab7294). Can be used as Capture antibody with recommended pair.


  • RelevanceIgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock.
  • Cellular localizationCell Membrane
  • Database links
  • Alternative names
    • Igh2 antibody
    • IGHE antibody
    • IGHEP1 antibody
    • Immunoglobulin heavy constant epsilon antibody

Anti-IgE antibody [5D4] images

  • Standard curve: Capture anitbody (ab7295) at 5 ug/ml in PBS, human IgE from 100 ng/ml to 1.56 ng/ml, detection antibody (ab7294, HRP conjugated) at 0.7 ug/ml concentration.

References for Anti-IgE antibody [5D4] (ab7295)

This product has been referenced in:

See 1 Publication for this product

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Thank you for contacting us.   I have received the following information from the lab: We are using ab7295 according to the datasheet recommendation as the capture antibody, not as detection antibody. The detection antibody ab7295 was used with the...

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Thank you for your enquiry. In our internal assay for anti-IgE MAb testing, highly purified myelomatous IgE(VL) was adsorbed on solid phase from solution (5 microgram/ml) in bicarbonate buffer pH 9.5. Ab7295 was applied for 60-120 min onto antige...

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Thank you for your enquiry and for completing our technical questionnaire. I am sorry to hear that you have been having difficulties with this antibody. I would greatly appreciate it if you could tell me of the concentration that you have been using...

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