Anti-IgE antibody [BE5] (Phycoerythrin) (ab77137)

Overview

  • Product nameAnti-IgE antibody [BE5] (Phycoerythrin)
    See all IgE primary antibodies
  • Description
    Mouse monoclonal [BE5] to IgE (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applicationsSuitable for: Flow Cyt, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) corresponding to Human IgE.

  • Positive control
    • Basophil from normal (heparin treated) whole blood stimulated with a combination of IL-3 and Goat anti-IgE polyclonal antibody.
  • General notes

    The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity>95% by SDS-PAGE
  • Purification notesThe conjugate is purified by size exclusion chromatography.
  • ClonalityMonoclonal
  • Clone numberBE5
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 5 - 10 µg/ml. ab91357-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ELISA Use at an assay dependent concentration.

Target

  • RelevanceIgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock.
  • Cellular localizationCell Membrane
  • Database links
  • Alternative names
    • Igh2 antibody
    • IGHE antibody
    • IGHEP1 antibody
    • Immunoglobulin heavy constant epsilon antibody

References for Anti-IgE antibody [BE5] (Phycoerythrin) (ab77137)

ab77137 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"