Overview

  • Product name
    Anti-IgE antibody, prediluted
    See all IgE primary antibodies
  • Description
    Rabbit polyclonal to IgE, prediluted
  • Specificity
    This antibody recognizes IgE, epsilon heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), mu (IgM), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes.
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length IgE isolated from a pool of normal human sera.

  • Positive control
    • Human Tonsil tissue
  • General notes


    This antibody is useful in the identification of leukemias, plasmacytomas and certain non-Hodgkin’s lymphomas.

Properties

  • Form
    Prediluted
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 15mM Sodium Azide
    Constituents: 0.5 mol/L Tris HCl, pH 7.6, and containing a stabilizing protein
  • Concentration information loading...
  • Purity
    Protein A purified
  • Primary antibody notes
    This antibody is useful in the identification of leukemias, plasmacytomas and certain non-Hodgkin’s lymphomas.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75673 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Ready to use.

Target

  • Relevance
    IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock.
  • Cellular localization
    Cell Membrane
  • Database links
  • Alternative names
    • Igh2 antibody
    • IGHE antibody
    • IGHEP1 antibody
    • Immunoglobulin heavy constant epsilon antibody

Images

  • Formalin-fixed, paraffin-embedded human tonsil stained with ab75673, using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of IgE-secreting cells.

References

This product has been referenced in:
  • Ningyan G  et al. The role of macrophage migration inhibitory factor in mast cell-stimulated fibroblast proliferation and collagen production. PLoS One 10:e0122482 (2015). Read more (PubMed: 25826375) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (duodenum - normal and celiac dz)
Specification
duodenum - normal and celiac dz
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA pH9.0, 100C 20 min
Permeabilization
No
Username

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Verified customer

Submitted Dec 09 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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