Overview

  • Product name
    Anti-IgE antibody [XTE4]
    See all IgE primary antibodies
  • Description
    Mouse monoclonal [XTE4] to IgE
  • Specificity
    Epsilon chain specific
  • Tested applications
    Suitable for: ELISA, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) corresponding to Human IgE. IgE from myelomatous human serum

  • General notes
    Concentration varies from lot to lot and can be provided on request.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 0.15M Sodium Chloride, 10mM Tris. pH 7.5
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    Purified by ion exchange chromatography, purity is tested by electrophoresis.
  • Clonality
    Monoclonal
  • Clone number
    XTE4
  • Myeloma
    Sp2/0
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7382 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1e+006. Can be used for the detection of total IgE level and IgE antibodies in ELISA.
WB Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution. PubMed: 21307287

Target

  • Relevance
    IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock.
  • Cellular localization
    Cell Membrane
  • Database links
  • Alternative names
    • Igh2 antibody
    • IGHE antibody
    • IGHEP1 antibody
    • Immunoglobulin heavy constant epsilon antibody

Anti-IgE antibody [XTE4] images

  • Anti-IgE antibody [XTE4] (ab7382) at 1 µg/ml + Natural human IgE protein (ab92394) at 0.01 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 8 minutes

References for Anti-IgE antibody [XTE4] (ab7382)

This product has been referenced in:
  • Palaniyandi S  et al. CD23-Dependent Transcytosis of IgE and Immune Complex across the Polarized Human Respiratory Epithelial Cells. J Immunol 186:3484-96 (2011). WB, ICC/IF, ELISA ; Human . Read more (PubMed: 21307287) »

See 1 Publication for this product

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In answer to your questions:

1. Yes, I believe the antibody ab7382 should be suitable for the experiment you have described, however, as we have not perfor...

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