- Datasheet
- Specific References (3)
- Protocols
Overview
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Product nameAnti-IGF1 antibody
See all IGF1 primary antibodies -
DescriptionRabbit polyclonal to IGF1
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, IHC-P, IHC (PFA fixed)more details
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Species reactivityReacts with: Mouse, Human
Predicted to work with: a wide range of other species
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Immunogen
Synthetic human insulin-like growth factor 1 [Ac60-70] covalently attached onto a carrier protein.
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Positive control
- This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: Hek293.
Properties
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FormLiquid
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Storage instructionsStore at +4°C short term (1-2 weeks) (add antibacterial agent). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: None
Constituents: Whole serum -
PurityWhole antiserum
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunohistochemistry kits
Applications
Our Abpromise guarantee covers the use of ab40657 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 1 µg/ml. | |
| IHC-P | 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| IHC (PFA fixed) | 1/1000. |
Target
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FunctionThe insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake.
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Involvement in diseaseDefects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation.
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Sequence similaritiesBelongs to the insulin family.
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Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 3479 Human
- Entrez Gene: 16000 Mouse
- Omim: 147440 Human
- SwissProt: P05019 Human
- SwissProt: P05017 Mouse
- Unigene: 160562 Human
- Unigene: 268521 Mouse
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FormThere are 2 isoforms produced by alternative splicing. Isoform 1 also known as: IGF-IB; Isoform 2 also known as: IGF-IA.
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Alternative names
- IBP1 antibody
- IGF I antibody
- IGF IA antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF1 antibody (ab40657)Image courtesy of Dr Lucila Sackmann Sala by Abreview.ab40657 staining IGF1 in murine prostate tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed with paraformaldehyde and an antigen retrieval step was performed using citrate buffer. Samples were then blocked with 1% serum for 20 minutes at 25°C and then incubated with ab40657 at a 1/1000 dilution for 18 hours at 4°C. The secondary used was a biotin conjugated horse polyclonal used at a 1/50 dilution. -
Immunocytochemistry/ Immunofluorescence - Anti-IGF1 antibody (ab40657)ab40657 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab40657 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF1 antibody (ab40657)This image is courtesy of an anonymous AbreviewImmunohistochemical analysis of rat colon tissue, staining IGF1 with ab40657.
Tissue was fixed with formaldehyde and blocked with 1% blocking solution for 15 minutes at room temperature; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/400 in BSA in TBS) for 1 hour. An undiluted HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.
References
This product has been referenced in:
- Thomsen J et al. PAPP-A proteolytic activity enhances IGF bioactivity in ascites from women with ovarian carcinoma. Oncotarget 6:32266-78 (2015). IHC . Read more (PubMed: 26336825) »
- Dhakal P et al. Neonatal Progesterone Programs Adult Uterine Responses to Progesterone and Susceptibility to Uterine Dysfunction. Endocrinology 156:3791-803 (2015). Read more (PubMed: 26204463) »
Customer reviews and Q&As
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