• Product nameAnti-IGF1 antibody
    See all IGF1 primary antibodies
  • Description
    Rabbit polyclonal to IGF1
  • Tested applicationsSuitable for: IHC-P, WB, Neutralising, ICC/IF, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Highly pure (>98%) recombinant hIGF-1 (human Insulin Like Growth Factor-1).



Our Abpromise guarantee covers the use of ab9572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. To detect hIGF-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Neutralising Use at an assay dependent concentration. To yield one-half maximal inhibition of the biological activity of hIGF-1 (5.0 ng/ml), a concentration of 0.67 - 1.0 ug/ml of this antibody is required.
ICC/IF 1/200.
Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to IGF1 (Biotin) (ab83137). Allows for the detection of at least 0.2 - 0.4 ng/well of recombinant IGF1.


  • FunctionThe insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake.
  • Involvement in diseaseDefects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation.
  • Sequence similaritiesBelongs to the insulin family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • FormThere are 2 isoforms produced by alternative splicing. Isoform 1 also known as: IGF-IB; Isoform 2 also known as: IGF-IA.
  • Alternative names
    • IBP1 antibody
    • IGF I antibody
    • IGF IA antibody
    • IGF IB antibody
    • IGF-I antibody
    • Igf1 antibody
    • IGF1_HUMAN antibody
    • IGF1A antibody
    • IGFI antibody
    • IGFIA antibody
    • Insulin like growth factor 1 (somatomedin C) antibody
    • Insulin like growth factor 1 antibody
    • Insulin like growth factor IA antibody
    • Insulin like growth factor IB antibody
    • Insulin-like growth factor I antibody
    • Mechano growth factor antibody
    • MGF antibody
    • OTTHUMP00000195080 antibody
    • OTTHUMP00000195081 antibody
    • OTTHUMP00000195082 antibody
    • OTTHUMP00000195083 antibody
    • OTTHUMP00000195084 antibody
    • Somatomedia C antibody
    • Somatomedin C antibody
    • Somatomedin-C antibody
    see all

Anti-IGF1 antibody images

  • Ab9572 staining Human normal liver parenchyma. Staining is localised to the cytoplasm.
    Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • ab9572 staining IGF1 in mouse heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% formalin and blocked with 10% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/1000 in 10% goat serum) for 24 hours at 4°C. An Alexa Fluor® 594-conjugated goat anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-IGF1 antibody (ab9572) at 1/2000 dilution

    Lane 1 : Human vascular smooth muscle whole cell lysate
    Lane 2 : Human vascular smooth muscle whole cell lysate
    Lane 3 : Human vascular smooth muscle whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Donkey Anti-Rabbit IgG - HRP at 1/10000 dilution
    Developed using the ECL technique

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ICC/IF image of ab9572 stained human embryonic stem cells. The cells were fixed in methanol and then incubated in 10% BSA for 1 hour to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9572, 1/200) overnight at +4°C. The secondary antibody (red) was an Alexa Fluor® 568 goat anti-rabbit used at a 1/400 dilution.

    See Abreview

References for Anti-IGF1 antibody (ab9572)

This product has been referenced in:
  • Nawathe AR  et al. Insulin-like growth factor axis in pregnancies affected by fetal growth disorders. Clin Epigenetics 8:11 (2016). WB ; Human . Read more (PubMed: 26823688) »
  • Ferraro ZM  et al. Excessive gestational weight gain and obesity contribute to altered expression of maternal insulin-like growth factor binding protein-3. Int J Womens Health 5:657-65 (2013). WB ; Human . Read more (PubMed: 24124394) »

See all 10 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (EL4 T cells tumor line and primary hepatocytes)
Permeabilization No
Gating Strategy Positive cells for FITC
Specification EL4 T cells tumor line and primary hepatocytes
Preparation Cell harvesting/tissue preparation method: Mice were dissected to obtain the liver. The liver was then mashed to obtain hepatocytes.
Sample buffer: PBS with 10% bovine serum
Fixation none

Safa Mohamad

Verified customer

Submitted May 12 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (mouse neuron)
Permeabilization Yes - triton X-100
Specification mouse neuron
Blocking step BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative Formaldehyde

Abcam user community

Verified customer

Submitted Dec 28 2015

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain)
Antigen retrieval step None
Specification Brain
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative Paraformaldehyde

Abcam user community

Verified customer

Submitted Apr 15 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step Heat mediated
Sample Mouse Tissue sections (Heart)
Specification Heart
Permeabilization No
Fixative 10% Formalin

Abcam user community

Verified customer

Submitted Jul 15 2014

To our knowledge, this IGF antibody has not been tested in flow cytometry and our guarantee does not cover this application of the antibody. However it has been tested and shown to work in a similar application, immunocytochemistry, after methanol fixa...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cultured Cells (human embryonic stem cells)
Specification human embryonic stem cells
Fixative Methanol
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C

Abcam user community

Verified customer

Submitted May 13 2013

Thank you for contacting us.

The lab sent me the following information:

The immunizing antigen was produced in E.coli.
The purification of this antibody is done using an antigen affinity column.
This is all the information we...

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Thank you for your message and for providing this further information.

To confirm, I can confirm this antibody has been tested and therefore guaranteed for human samples. The previous email stated that it has not been tested specifically wit...

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Thank you for your reply

Please note that theseantibodies have not been tested with with serum samples, and so I am sorry no pretreatments have been tested which we could provide you with information on. In theory the antibodiesshould work w...

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Thank you for your reply.

I would like to reassure you that ab83137 Anti-IGF1 antibody (Biotin) and ab9572 Anti-IGF1 antibody are tested and covered by our 6 month guarantee for sandwich ELISA together, and human samples. In the event that a...

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